Pancreatic juice collected by endoscopic retrograde cannulation of the pancreatic duct after administration of secretin and pancreozymin was analyzed by pore gradient polyacrylamide gel electrophoresis. Two electrophoretic systems, an alkaline (pH 8.9) and an acidic (pH 5.0), were elaborated to obtain optimal separation of exocrine proteins. 13 bands under alkaline conditions and 17 bands by running in the acidic system were separated and distinguished by Coomassie-Brilliant Blue G 250 staining. Fourteen proteins were identified using enzymatic and immunological methods: one form of each of lipase, chymotrypsinogen, prophospholipase A2, procarboxypeptidase A and trypsin inhibitor, two forms of each of procarboxypeptidase B and proelastase, three forms of each of trypsinogen and amylase. An additional band was identified as albumin.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Hydroxyapatite Chitosan Gradient Pore Scaffold Activates Oxidative Phosphorylation Pathway to Induce Bone Formation.
Front Biosci (Landmark Ed)
January 2025
Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Fujian Medical University, Fujian Provincial Key Laboratory of Stomatology, National Regional Medical Center, Binhai Campus of The First Affiliated Hospital, 350005 Fuzhou, Fujian, China.
Background: In this study, we prepared a porous gradient scaffold with hydroxyapatite microtubules (HAMT) and chitosan (CHS) and investigated osteogenesis induced by these scaffolds.
Methods: The arrangement of wax balls in the mold can control the size and distribution of the pores of the scaffold, and form an interconnected gradient pore structure. The scaffolds were systematically evaluated and for biocompatibility, biological activity, and regulatory mechanisms.
Pulsatile Ion Transport in Nanofiltration Membranes Coupled with Electrically Tunable Pore and Hydroxyl Electrostatic Interactions.
ACS Nano
January 2025
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Pulsatile ion transport facilitates the adjusted transfer of substances, meeting the requirements for the gradient and timed separation of multiple components in membrane processes. Responsive nanofiltration membranes are thus currently receiving widespread attention but face limitations due to their narrow performance adjustment range. Herein, hydroxyl functional groups were introduced into electrically responsive nanofiltration membranes to broaden the adjustment range of separation performance through a combination of pore size sieving and functional group interactions, resulting in a greater change in rejection and flux compared to the original membrane.
View Article and Find Full Text PDFSolvothermal Template-Induced Hierarchical Porosity in Covalent Organic Frameworks: A Pathway to Enhanced Diffusivity.
Adv Mater
January 2025
Max Planck Institute for Solid State Research, Heisenbergstr. 1, 70569, Stuttgart, Germany.
The rapid advancement of covalent organic frameworks (COFs) in recent years has firmly established them as a new class of molecularly precise and highly tuneable porous materials. However, compared to other porous materials, such as zeolites and metal-organic frameworks, the successful integration of hierarchical porosity into COFs remains largely unexplored. The challenge lies in identifying appropriate synthetic methods to introduce secondary pores without compromising the intrinsic structural porosity of COFs.
View Article and Find Full Text PDFShear thickening inside elastic open-cell foams under dynamic compression.
Soft Matter
January 2025
James Franck Institute and Department of Physics, The University of Chicago, Chicago, Illinois 60637, USA.
We measure the response of open-cell polyurethane foams filled with a dense suspension of fumed silica particles in polyethylene glycol at compression speeds spanning several orders of magnitude. The gradual compressive stress increase of the composite material indicates the existence of shear rate gradients in the interstitial suspension caused by wide distributions in pore sizes in the disordered foam network. The energy dissipated during compression scales with an effective internal shear rate, allowing for the collapse of three data sets for different pore-size foams.
View Article and Find Full Text PDFNeuron-inspired CsPbBr/PDMS nanospheres for multi-dimensional sensing and interactive displays.
Light Sci Appl
January 2025
National and Local United Engineering Laboratory of Flat Panel Display Technology, College of Physics and Information Engineering, Fuzhou University, 350108, Fuzhou, China.
Multifunctional materials have attracted tremendous attention in intelligent and interactive devices. However, achieving multi-dimensional sensing capabilities with the same perovskite quantum dot (PQD) material is still in its infancy, with some considering it currently challenging and even unattainable. Drawing inspiration from neurons, a novel multifunctional CsPbBr/PDMS nanosphere is devised to sense humidity, temperature, and pressure simultaneously with unique interactive responses.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!