The plant hormone auxin plays a central role in adventitious rooting and is routinely used with many economically important, vegetatively propagated plant species to promote adventitious root initiation and development on cuttings. Nevertheless the molecular mechanisms through which it acts are only starting to emerge. The Arabidopsis superroot2-1 (sur2-1) mutant overproduces auxin and, as a consequence, develops excessive adventitious roots in the hypocotyl. In order to increase the knowledge of adventitious rooting and of auxin signalling pathways and crosstalk, this study performed a screen for suppressors of superroot2-1 phenotype. These suppressors provide a new resource for discovery of genetic players involved in auxin signalling pathways or at the crosstalk of auxin and other hormones or environmental signals. This study reports the identification and characterization of 26 sur2-1 suppressor mutants, several of which were identified as mutations in candidate genes involved in either auxin biosynthesis or signalling. In addition to confirming the role of auxin as a central regulator of adventitious rooting, superroot2 suppressors indicated possible crosstalk with ethylene signalling in this process.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967091PMC
http://dx.doi.org/10.1093/jxb/eru026DOI Listing

Publication Analysis

Top Keywords

adventitious rooting
16
auxin signalling
8
signalling pathways
8
pathways crosstalk
8
involved auxin
8
auxin
7
adventitious
5
identification adventitious
4
rooting
4
rooting mutants
4

Similar Publications

Willows (genus ) are increasingly used in operational-scale ecosystem reclamation; however, different opinions exist regarding the optimal cutting size for planting under field conditions. We compared the survival of field-planted willow cuttings sourced from upland and lowland areas with varying diameters and lengths across two growing seasons. Cuttings were grouped into 15 size classes with different diameters (0.

View Article and Find Full Text PDF

In Vitro Rooting of Poplar: Effects and Metabolism of Dichlorprop Auxin Ester Prodrugs.

Plants (Basel)

January 2025

Laboratory for Applied In Vitro Plant Biotechnology, Ghent University, 9000 Ghent, Belgium.

Efficient adventitious root formation is essential in micropropagation. Auxin prodrugs, inactive precursors that convert into active auxins within the plant, offer potentially improved rooting control and reduced phytotoxicity. This study investigated the efficacy of dichlorprop ester (DCPE), commercialized as Corasil and Clemensgros (originally intended to increase grapefruit size), in promoting in vitro root initiation in the model plant × , compared to its hydrolyzed form DCP and the related compound C77.

View Article and Find Full Text PDF

Depletion of Gibberellin Signaling Up-Regulates Transcription and Promotes Adventitious Root Formation in Leaf Explants.

Int J Mol Sci

December 2024

Jiangxi Provincial Key Laboratory of Plant Germplasm Innovation and Genetic Improvement, Lushan Botanical Garden, Chinese Academy of Sciences, Jiujiang 332900, China.

Adventitious root (AR) formation in plants originates from non-root organs such as leaves and hypocotyls. Auxin signaling is essential for AR formation, but the roles of other phytohormones are less clear. In , at least two distinct mechanisms can produce ARs, either from hypocotyls as part of the general root architecture or from wounded organs during de novo root regeneration (DNRR).

View Article and Find Full Text PDF

De novo shoot regeneration, characterized by the emergence of adventitious shoots from excised or damaged tissues or organs in vitro, is regulated by the complex interplay between genetic and epigenetic regulatory mechanisms. However, the specific effect of histone deacetylation on shoot regeneration remains poorly understood. This study investigated the effects of trichostatin A (TSA), a histone deacetylase inhibitor, on shoot regeneration in callus derived from root explants.

View Article and Find Full Text PDF

A root system architecture regulator modulates OsPIN2 polar localization in rice.

Nat Commun

January 2025

State Key Laboratory of Plant Environmental Resilience, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.

Ideal root system architecture (RSA) is important for efficient nutrient uptake and high yield in crops. We cloned and characterized a key RSA regulatory gene, GRAVITROPISM LOSS 1 (OsGLS1), in rice (Oryza sativa L.).

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!