Biomarkers of oxidative stress in acute and chronic bronchial asthma.

Objective: Oxidative stress plays an important role in inflammatory diseases of the airways, such as bronchial asthma. This study highlights the effects of the imbalance of oxidant/antioxidant parameters and their roles in the severity of asthma, in order to target the deficiency of antioxidants when treating asthmatic patients.

Methods: The study included 45 male and 45 female subjects, aged 18-48 years, with and without asthma. They were classified into 3 groups: G1 was healthy volunteers (CT, n = 15), G2 included patients with stable (chronic) conditions of asthma from the outpatients department (OP, n = 15), and G3 included patients admitted emergency room (ER, n = 15) with acute asthma attacks. For all subjects, the levels of total antioxidant capacity (TAC), reduced glutathione (GSH), malondialdehyde (MDA), and uric acid were colorimetrically estimated. The correlations between the studied parameters were statistically analyzed.

Results: The levels of TAC and GSH in asthmatic patients, either male or female, were significantly decreased in the ER more than in the OP group as compared to the control (p < 0.001). MDA increased significantly in the ER group and over the OP group when compared with healthy subjects. Significant positive correlations (p < 0.001) were observed between MDA and other studied parameters TAC (r = -0.74), GSH (r = -0.69), and uric acid (r = -0.35).

Conclusions: The levels of total antioxidants were inversely related to the severity of asthma as observed in asthmatic patients visiting outpatient clinics and patients with acute asthmatic attacks admitted to emergency rooms. Meanwhile, those patients with acute attacks showed increased oxidative stress as reflected in the raised MDA levels.