A large body of evidence has linked retinol-binding protein 4 (RBP4) to systemic insulin resistance, but little is known about its function in fat deposition. This study aimed to confirm the involvement of RBP4 in inguinal fat deposition and insulin by intraperitoneal injection of adenovirus-mediated RBP4 to mice. Intraperitoneal injection of adenoviral vectors was validated as an efficient gene manipulation tool for over-expressing recombinant proteins in vivo. Ectopic expression of RBP4 decelerated inguinal fat deposition by decreasing the size of adipocytes. Moreover, the introduction of exogenous RBP4 blunted the response of inguinal adipocytes to insulin signals. These findings suggest that RBP4 impaired in vivo adipogenesis, partly through the repression of the insulin pathway.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s13105-014-0326-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of implant status and breed type on feedlot performance, carcass characteristics, sera metabolites, and immunohistochemical responses in finishing steers.
J Anim Sci
January 2025
Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX USA.
The number of beef × dairy animals entering feedlots has increased, but the response of beef × dairy cattle to growth-promoting implants has not been well characterized. The objective of this study was to evaluate the effects of breed type and implant administration on live performance, carcass characteristics, sera metabolites, and immunohistochemical (IHC) outcomes. Forty-eight steers (average body weight [BW] = 417±22 kg) were sorted by breed into groups of predominantly Angus (B), black-hided beef × primarily Holstein (B×D), or Holstein (D), and half of the steers within each breed type were administered a steroidal implant.
View Article and Find Full Text PDFSerum markers for beef meat quality: Potential media supplement for cell-cultured meat production.
Curr Res Food Sci
December 2024
Department of Medical Biotechnology and Research Institute of Cell Culture, Yeungnam University, Gyeongsan, 38541, Republic of Korea.
As the global population continues to grow and food demands increase, the food industry faces mounting pressure to develop innovative solutions. Cell-cultured meat involves cultivating cells from live animals through self-renewal methods or scaffolding and presents a promising alternative to traditional meat production by generating nutritionally rich biomass. However, significant research is still needed to overcome challenges such as developing serum-free media, identifying suitable additives to support cell growth, and ensuring the quality of cell-cultured meat closely resembles that of traditional meat.
View Article and Find Full Text PDFBenzopyrene Aggravates Nonalcoholic Liver Fatty Diseases in Female Mice Via the AHR/ERα Axis.
Curr Mol Med
January 2025
Inflammation and Immune-Mediated Diseases Laboratory of Anhui Province, School of Pharmacy, Anhui Medical University, Hefei, Anhui, China.
Article Synopsis
- The study investigates how Benzo[a]pyrene (BaP), an environmental pollutant, affects the progression of nonalcoholic fatty liver disease (NAFLD) through its interaction with the AHR/ERα axis.
- In experiments using high-fat diet models and Oleic acid treatment on HepG2 cells and C57BL/6J mice, exposure to BaP was found to inhibit ERα protein levels and disrupt lipid metabolism, evidenced by changes in key biomarkers (CYP1A1, SREBP-1c).
- The presence of estradiol (E2) can mitigate some negative effects of Oleic acid on lipid accumulation; however, BaP reduces the beneficial effects of E
Small molecule-driven LKB1 deacetylation is responsible for the inhibition of hepatic lipid response in NAFLD.
J Lipid Res
January 2025
Institute of Endocrine and Metabolic Diseases, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China. Electronic address:
Nonalcoholic fatty liver disease (NAFLD) is a progressive condition characterized by ectopic fat accumulation in the liver, for which no FAD-approved drugs currently exist. Emerging evidence highlights the role of liver kinase B1 (LKB1), a key metabolic regulator, has been proposed in NAFLD, particularly in response to excessive nutrient levels. However, few agents have been identified that can prevent the progression of nonalcoholic steatohepatitis (NASH) by targeting LKB1 deacetylation.
View Article and Find Full Text PDFImpact of particle size on and fat fraction estimation for accurate assessment of hepatic iron overload and steatosis using MRI.
Magn Reson Med
January 2025
Department of Biomedical Engineering, The University of Memphis, Memphis, Tennessee, USA.
Purpose: To investigate the impact of iron particle size on and fat fraction (FF) estimations for coexisting hepatic iron overload and steatosis condition using Monte Carlo simulations and phantoms.
Methods: Three iron particle sizes (0.38, 0.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!