A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 176

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 1034
Function: getPubMedXML

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3152
Function: GetPubMedArticleOutput_2016

File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

MYB gene abnormalities t(6;9) in adenoid cystic carcinoma fine-needle aspiration biopsy using fluorescence in situ hybridization. | LitMetric

MYB gene abnormalities t(6;9) in adenoid cystic carcinoma fine-needle aspiration biopsy using fluorescence in situ hybridization.

Arch Pathol Lab Med

From the Department of Pathology and Immunology, St Louis School of Medicine, Washington University, St Louis, Missouri.

Published: March 2014

Context: Fine-needle aspiration (FNA) biopsy of salivary gland neoplasms can have a variety of overlapping appearances. Basaloid neoplasms can be a diagnostic challenge, and FNA cytomorphology alone cannot always provide a definitive diagnosis.

Objective: To examine the incidence and potential utility of detecting a MYB translocation by fluorescence in situ hybridization (FISH) in adenoid cystic carcinomas (AdCCs) and pleomorphic adenoma FNA smears with known surgical outcomes.

Design: Patients who underwent FNA biopsy for surgically confirmed AdCCs and pleomorphic adenomas were identified. Fluorescence in situ hybridization, using commercially available fluorescent-labeled probes, hybridizing to MYB-telomeric and MYB-centromeric, was used to identify the MYB gene and to evaluate it for abnormalities and translocation. Using a fluorescent microscope, 4',6-diamidino-2-phenylindole (DAPI)-stained, nonoverlapping cells were counted, and 10% or greater abnormal cells were considered positive.

Results: The 10 AdCC and 13 pleomorphic adenoma FNA cases had FISH evaluations performed; 50% (5 of 10) of the AdCC cases showed a MYB abnormality by FISH; 40% (4 of 10) AdCCs showed a positive break-apart signal in most cells (48%-84%). One case (10%) of AdCC showed a trisomy MYB signal pattern without the break-apart translocation pattern. Of the 13 pleomorphic adenomas, none (0%) of the cases showed a MYB translocation or abnormality by FISH. MYB FISH abnormalities showed a 100% positive predictive value, 50% sensitivity, and 100% specificity, when differentiating AdCC from pleomorphic adenoma.

Conclusions: MYB gene abnormalities were present in 50% (5 of 10) of the AdCC cases. This corresponds to the reported prevalence in formalin-fixed, paraffin-embedded tissue for AdCC surgical resections. Using FISH testing for detecting MYB gene abnormalities in the salivary gland of FNA biopsies has the potential to provide additional, helpful ancillary information in diagnosing AdCC.

Download full-text PDF

Source
http://dx.doi.org/10.5858/arpa.2012-0736-OADOI Listing

Publication Analysis

Top Keywords

myb gene
16
gene abnormalities
12
fluorescence situ
12
situ hybridization
12
myb
9
adenoid cystic
8
fine-needle aspiration
8
fna biopsy
8
salivary gland
8
detecting myb
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!