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Genome-wide association mapping of acute lung injury in neonatal inbred mice. | LitMetric

Genome-wide association mapping of acute lung injury in neonatal inbred mice.

FASEB J

Laboratory of Respiratory Biology, National Institute of Environmental Health Sciences, U.S. National Institutes of Health, Research Triangle Park, North Carolina, USA;

Published: June 2014

AI Article Synopsis

  • - Reactive oxygen species (ROS) are linked to respiratory disorders like bronchopulmonary dysplasia in preterm infants, but the exact reasons why neonatal lungs are vulnerable to oxidant stress are not fully known.
  • - Researchers studied 36 different mouse strains exposed to high oxygen levels to see how their genetic backgrounds affected lung injury, discovering significant variations in inflammatory responses.
  • - A specific gene, Chrm2, was identified as influencing susceptibility to hyperoxia-induced lung damage, with mice lacking this gene or having a protective genetic mutation showing less inflammation compared to others.

Article Abstract

Reactive oxygen species (ROS) contribute to the pathogenesis of many acute and chronic pulmonary disorders, including bronchopulmonary dysplasia (BPD), a respiratory condition that affects preterm infants. However, the mechanisms of susceptibility to oxidant stress in neonatal lungs are not completely understood. We evaluated the role of genetic background in response to oxidant stress in the neonatal lung by exposing mice from 36 inbred strains to hyperoxia (95% O2) for 72 h after birth. Hyperoxia-induced lung injury was evaluated by using bronchoalveolar lavage fluid (BALF) analysis and pathology. Statistically significant interstrain variation was found for BALF inflammatory cells and protein (heritability estimates range: 33.6-55.7%). Genome-wide association mapping using injury phenotypes identified quantitative trait loci (QTLs) on chromosomes 1, 2, 4, 6, and 7. Comparative mapping of the chromosome 6 QTLs identified Chrm2 (cholinergic receptor, muscarinic 2, cardiac) as a candidate susceptibility gene, and mouse strains with a nonsynonymous coding single-nucleotide polymorphism (SNP) in Chrm2 that causes an amino acid substitution (P265L) had significantly reduced hyperoxia-induced inflammation compared to strains without the SNP. Further, hyperoxia-induced lung injury was significantly reduced in neonatal mice with targeted deletion of Chrm2, relative to wild-type controls. This study has important implications for understanding the mechanisms of oxidative lung injury in neonates.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4021442PMC
http://dx.doi.org/10.1096/fj.13-247221DOI Listing

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