The hypothesis that a wild-type strain of Xylella fastidiosa would restore the ability of rpfF mutants blocked in diffusible signal factor production to be transmitted to new grape plants by the sharpshooter vector Graphocephala atropunctata was tested. While the rpfF mutant was very poorly transmitted by vectors irrespective of whether they had also fed on plants infected with the wild-type strain, wild-type strains were not efficiently transmitted if vectors had fed on plants infected with the rpfF mutant. About 100-fewer cells of a wild-type strain attached to wings of a vector when suspended in xylem sap from plants infected with an rpfF mutant than in sap from uninfected grapes. The frequency of transmission of cells suspended in sap from plants that were infected by the rpfF mutant was also reduced over threefold. Wild-type cells suspended in a culture supernatant of an rpfF mutant also exhibited 10-fold less adherence to wings than when suspended in uninoculated culture media. A factor released into the xylem by rpfF mutants, and to a lesser extent by the wild-type strain, thus inhibits their attachment to, and thus transmission by, sharpshooter vectors and may also enable them to move more readily through host plants.
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http://dx.doi.org/10.1094/PHYTO-06-13-0151-R | DOI Listing |
Microorganisms
December 2024
School of Chemistry, University College Cork, T12 YN60 Cork, Ireland.
is an aerobic, Gram-negative bacterium that is responsible for many plant diseases. The bacterium is the causal agent of Pierce's disease in grapes and is also responsible for citrus variegated chlorosis, peach phony disease, olive quick decline syndrome and leaf scorches of various species. The production of biofilm is intrinsically linked with persistence and transmission in .
View Article and Find Full Text PDFPhytopathology
January 2025
Guangxi Key Laboratory for Sugarcane Biology & State Key Laboratory of Conservation and Utilization for Subtropical Agri-Biological Resources, Guangxi University, Nanning, Guangxi, 530005, China.
spp. are plant pathogens known for significantly impacting crop yields. Among them, () is notable for colonizing the xylem and causing sugarcane leaf scald disease.
View Article and Find Full Text PDFFront Microbiol
April 2023
CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
Quorum sensing (QS) system has an essential function in plant growth-promoting rhizobacteria (PGPR) response to environmental stress and PGPR induction of plant tolerance to saline-alkaline stress. Nevertheless, there is a lack of understanding about how QS influences the growth-promoting effects of PGPR on plants. DSM14405 is a PGPR with a QS system, which can secrete diffusible signal factor (DSF), one of the QS signal molecules.
View Article and Find Full Text PDFNPJ Biofilms Microbiomes
November 2022
Department of Microbiology, University of Zürich, Zürich, Switzerland.
The polysaccharide Bep is essential for in vitro biofilm formation of the opportunistic pathogen Burkholderia cenocepacia. We found that the Burkholderia diffusible signaling factor (BDSF) quorum sensing receptor RpfR is a negative regulator of the bep gene cluster in B. cenocepacia.
View Article and Find Full Text PDFSci Total Environ
August 2022
CAS Key Laboratory of Environment Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China; Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China. Electronic address:
Quorum sensing (QS) regulates various physiological processes in a cell density-dependent mode via cell-cell communication. Stenotrophomonas rhizophila DSM14405 having the diffusible signal factor (DSF)-QS system, is a plant growth-promoting rhizobacteria (PGPR) that enables host plants to tolerate saline-alkaline stress. However, the regulatory mechanism of DSF-QS in S.
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