[Combined effects of T-2 toxin and selenium deficiency on lipid peroxidation in rat's brain].

Objective: To explore the effects of T-2 toxin and it's synergy with low selenium on lipid peroxidation in rats' brain.

Methods: Early weaning male SD (Sprague-Dawley) rats were randomly divided into normal group and low-selenium model group by weight after 1 week for adaptive feeding, and respectively fed normal diet and low-selenium diet. After the low Se model was succeed, the normal group were randomly divided into control group, low T-2 group, high T-2 group, the model group were randomly divided into low Se group, low Se + low T-2 group, low Se + high T-2 group. Then give T-2 toxin to the low T-2 group, low Se + low T-2 group at the dose of 0.1 mg/(kg x d), and high T-2 group, low Se + high T-2 group at the dose of 0.2 (kg x d), intragastric administration for 4 weeks. We take the brain sample, observed the morphological changes of brain tissue after HE staining, measured the content of MDA and the activity of GSH-Px to observe the T-2 toxin' effect of antioxidant on rat brain.

Results: The activity of rats' brain GSH-Px in all low Se groups were lower than the control group (P < 0.05). The content of rats' brain MDA in all experimental group were higher than the control group (P < 0.05). In all experimental group, the structure, the neurons, the nuclei of rats' brain tissue have varying degrees of damage and destruction.

Conclusion: The single factor of T-2 toxin can cause lipid peroxidation in brain, lower the activity of GSH-Px and higher the lever of MDA. The single factor of low Se can induce lipid peroxidation in rats' brain, lower the activity of GSH-Px, higher the level of MDA, With the condition of low Se, the effect of T-2 toxin on lipid peroxidation in rats' brain was more obviously than the single factor. There have synergistic effect T-2 toxin with low Se.