AI Article Synopsis
- Zebrafish are an important model organism for research in development, disease modeling, and drug screening due to their vertebrate characteristics.
- New genetic tools have been developed for zebrafish, but traditional genotyping methods are overly time-consuming and labor-intensive, hindering large-scale research.
- A new technique using PCR and high-resolution melting analysis (HRMA) offers a faster, more sensitive, and cost-effective method for genotyping zebrafish, suitable for both embryos and adult fish, facilitating high-throughput screening.
Article Abstract
Zebrafish is a powerful vertebrate model system for studying development, modeling disease, and performing drug screening. Recently a variety of genetic tools have been introduced, including multiple strategies for inducing mutations and generating transgenic lines. However, large-scale screening is limited by traditional genotyping methods, which are time-consuming and labor-intensive. Here we describe a technique to analyze zebrafish genotypes by PCR combined with high-resolution melting analysis (HRMA). This approach is rapid, sensitive, and inexpensive, with lower risk of contamination artifacts. Genotyping by PCR with HRMA can be used for embryos or adult fish, including in high-throughput screening protocols.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4116811 | PMC |
| http://dx.doi.org/10.3791/51138 | DOI Listing |
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