AI Article Synopsis
- The study evaluated biofilm formation in non-typeable Haemophilus influenzae (NTHi) and H. influenzae type b (Hib) clinical isolates using serotyping and PCR techniques to classify strains into four groups based on their resistance to ampicillin.
- A total of 99 strains were analyzed, with the gBLNAS, gBLNAR, and gBLPAR-NTHi groups showing higher biofilm production compared to Hib strains, as measured by OD readings in microtiter assays and visual observation using SEM and CLSM.
- The results indicated that resistance alone isn't the primary factor influencing biofilm formation; rather, the presence of a capsule on certain strains appears to enhance biofilm production in H.
Article Abstract
To evaluate the biofilm formation of non-typeable Haemophilus influenzae (NTHi) and H. influenzae type b (Hib) clinical isolates, we conducted the following study. Serotyping and polymerase chain reaction were performed to identify β-lactamase-negative ampicillin (ABPC)-susceptible (BLNAS), β-lactamase-negative ABPC-resistant (BLNAR), TEM-1 type β-lactamase-producing ABPC-resistant (BLPAR)-NTHi, and Hib. Biofilm formation was investigated by microtiter biofilm assay, as well as visually observation with a scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) in a continuous-flow chamber. As a result, totally 99 strains were investigated, and were classified into 4 groups which were 26 gBLNAS, 22 gBLNAR, 28 gBLPAR-NTHi and 23 Hib strains. The mean OD600 in the microtiter biofilm assay of gBLNAS, gBLNAR, gBLPAR-NTHi, and Hib strains were 0.57, 0.50, 0.34, and 0.08, respectively. NTHi strains were similar in terms of biofilm formations, which were observed by SEM and CLSM. Five Hib strains with the alternated type b cap loci showed significantly increased biofilm production than the other Hib strains. In conclusion, gBLNAS, gBLNAR, and gBLPAR-NTHi strains were more capable to produce biofilms compared to Hib strains. Our data suggested that resistant status may not be a key factor but capsule seemed to play an important role in H. influenzae biofilm formation.
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| http://dx.doi.org/10.1016/j.jiac.2013.06.001 | DOI Listing |
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