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| http://dx.doi.org/10.1016/j.bpj.2014.01.019 | DOI Listing |
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Selective deuteration of an RNA:RNA complex for structural analysis using small-angle scattering.
bioRxiv
September 2024
Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, USA.
The structures of RNA:RNA complexes regulate many biological processes. Despite their importance, protein-free RNA:RNA complexes represent a tiny fraction of experimentally-determined structures. Here, we describe a joint small-angle X-ray and neutron scattering (SAXS/SANS) approach to structurally interrogate conformational changes in a model RNA:RNA complex.
View Article and Find Full Text PDFScrutinizing the protein hydration shell from molecular dynamics simulations against consensus small-angle scattering data.
Commun Chem
December 2023
Theoretical Physics and Center for Biophysics, Saarland University, Saarbrücken, 66123, Germany.
Biological macromolecules in solution are surrounded by a hydration shell, whose structure differs from the structure of bulk solvent. While the importance of the hydration shell for numerous biological functions is widely acknowledged, it remains unknown how the hydration shell is regulated by macromolecular shape and surface composition, mainly because a quantitative probe of the hydration shell structure has been missing. We show that small-angle scattering in solution using X-rays (SAXS) or neutrons (SANS) provide a protein-specific probe of the protein hydration shell that enables quantitative comparison with molecular simulations.
View Article and Find Full Text PDFPhase behaviour and aggregate structures of the surface-active ionic liquid [BMIm][AOT] in water.
J Colloid Interface Sci
December 2023
School of Molecular Sciences, The University of Western Australia, Perth, Western Australia, Australia. Electronic address:
Hypothesis: The surface-active ionic liquid, 1-butyl-3-methylimidazolium 1,4-bis-2-ethylhexylsulfosuccinate ([BMIm][AOT]), has a sponge-like bulk nanostructure consisting of percolating polar and apolar domains formed by the ion charge groups and alkyl chains, respectively. We hypothesise that added water will swell the polar domains and change the liquid nanostructure.
Experiments: Small angle X-ray scattering (SAXS), small angle neutron scattering (SANS) and polarizing optical microscopy (POM) were used to investigate the nanostructure of [BMIm][AOT] as a function of water content.
Characterization of Zr-Containing Dispersoids in Al-Zn-Mg-Cu Alloys by Small-Angle Scattering.
Materials (Basel)
January 2023
Chair of Physics, Department Physics, Mechanics and Electrical Engineering, Montanuniverstät Leoben, Franz-Josef-Str. 18, 8700 Leoben, Austria.
Article Synopsis
- Characterizing Zr-containing dispersoids in aluminum alloys is tough due to their size variability and uneven distribution in the material.
- This study compared small-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS) with scanning electron microscopy (SEM) and transmission electron microscopy (TEM) for analyzing these dispersoids in a specific type of aluminum alloy.
- SAXS was found to be more sensitive to Zr dispersoids, while SANS can examine larger sample volumes; using both techniques together effectively distinguishes dispersoids from other phases, although SEM results diverged from others due to its resolution limits.
A novel experimental approach for nanostructure analysis: simultaneous small-angle X-ray and neutron scattering.
J Appl Crystallogr
June 2020
Institute for Crystallography and Structural Physics, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstrasse 3, Erlangen, 91058, Germany.
Exploiting small-angle X-ray and neutron scattering (SAXS/SANS) on the same sample volume at the same time provides complementary nanoscale structural information in two different contrast situations. Unlike an independent experimental approach, the truly combined SAXS/SANS experimental approach ensures the exactness of the probed samples, particularly for studies. Here, an advanced portable SAXS system that is dimensionally suitable for installation in the D22 zone of ILL is introduced.
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