[Chinese medicine formula 1 regulating immunity and anti-oxidation experiments in mice].

Objective: To observe the effect of immunoregulation and anti-oxidation of Zhongyaofangji NO1 (ZYFJ).

Methods: 1. SPF BALB/C mice were randomly divided into control group (distilled water), positive control group (Broken Spore), ZYFJ low dose group (0.35 g/kg), middle dose group (0.70 g/kg) and high dose group (1.40 g/kg), with intragastric administration 1 time/d for 30d; The spleen and thymus index, ability of spleen lymphocyte proliferation, phagocytosing chicken red blood cell (CRBC) of abdominal macrophage cell, carbon clearance were investigated. 2. SPF BALB/C mice were divided into normal control group, model control group (D-galactose induced peroxidation damage), model plus ZYFJ low dose, middle dose and high dose group, model plus positive control group (Broken Spore), with drug administration 1 time/d for 30 d. SOD activity, MDA and LPO content in brain tissue were tested while Nrf2 [Nuclear factor (erythroi D-derived 2)-like 2] protein expression in brain tissue nucleus was tested by western blotting.

Results: The thymus index and spleen index in groups of ZYFJ high dose and positive control were higher than those in control group, the ability of lymphocyte proliferation and phagocytosis of macrophages were increased in all the other groups significantly compared with control group; The activity of SOD and Nrf2 protein expression level in brain tissue of model mice was increased, MDA and LPO contents were reduced in ZYFJ middle and high dose as well as positive control significantly, while the MDA content was reduced and Nrf2 protein was increased in low dose group.

Conclusion: Appropriate dose of ZYFJ1 has good effect of immunoregulation, and plays a role of anti-oxidation probably by regulating Nrf2 protein expression in brain tissue and related signaling pathway.