A bio-inspired in vitro disease model was developed to investigate the basic biophysics of atherosclerotic diseases. In vivo study was conducted in advance using zebrafish fed with a normal diet and a cholesterol-enriched diet. The endothelial cells (ECs) of the zebrafishes fed with a normal diet are tightly attached and aligned. Their collagen has a fiber-like structure. By contrast, the endothelial cells of the zebrafish on high cholesterol diet are disorganized and their collagen has broken structures. In vitro models of human umbilical vein endothelial cells (HUVECs) were established on collagen films to mimic such in vivo experimental results. The normal collagen film simulates the extracellular matrix (ECM) in the blood vessels of a normal zebrafish, and the collagenase-treated collagen film mimics the ECM in blood vessels of an abnormal zebrafish. The HUVECs cultured on the normal collagen film are tightly attached, similar to those of a normal zebrafish. However, the cells cultured on the collagenase-treated collagen film are aggregated and biomarkers of endothelial dysfunction are expressed on the surface of the abnormal endothelial cells established on the denatured collagen film. The present in vitro model using a bio-inspired collagen film has a great potential for the design of novel therapies for clinical treatments of atherosclerosis through better understanding on the outbreak mechanism of atherosclerosis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.mvr.2014.02.003 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Enhanced Antitumor Efficacy and Reduced Toxicity in Colorectal Cancer Using a Novel Multifunctional Rg3- Targeting Nanosystem Encapsulated with Oxaliplatin and Calcium Peroxide.
Int J Nanomedicine
January 2025
Department of Biopharmacy, School of Pharmaceutical Sciences, Jilin University, Changchun, People's Republic of China.
Article Synopsis
- Colorectal cancer (CRC) is a major cause of cancer deaths, and oxaliplatin (OXA) is a primary treatment that faces challenges due to the tumor microenvironment (TME).
- A new multifunctional nanosystem, Rg3-Lip-OXA/CaO, uses Ginsenoside Rg3 liposomes to target CRC cells, delivering OXA and calcium peroxide (CaO) together.
- Research showed that this nanosystem had good stability and release properties, effectively targeted cancer cells, and significantly suppressed tumor growth in mice, while also showing manageable acute toxicity.
Keratin/chitosan film promotes wound healing in rats with combined radiation-wound injury.
J Mater Sci Mater Med
January 2025
Department of Nuclear Medicine, Chongqing University Cancer Hospital, No. 181 HanYu St, Shapingba District, Chongqing, 400030, PR China.
Human hair keratin, a natural protein derived from human hair, has emerged prominently in the field of wound repair, showcasing its unique regenerative capabilities and extensive application potential. However, it is a challenge for the keratin to efficiently therapy the impaired wound healing, such as combined radiation-wound injury. Here, we report a keratin/chitosan (KRT/CS) film for skin repair of chronic wounds in in rats with combined radiation-wound injury.
View Article and Find Full Text PDFStreptococcus pyogenes M1UK Variant-Associated Sartorius Muscle Necrotizing Soft Tissue Infection: A Case Report and Literature Review.
Cureus
December 2024
Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences & Ōmura Satoshi Memorial Institute, Kitasato University, Tokyo, JPN.
Necrotizing soft tissue infections (NTSIs) represent a concept of necrotizing infections involving the skin, subcutaneous tissue, fascia, and muscle, and it is a potentially fatal disease. Early exploratory incision is strongly recommended for both the diagnosis and treatment of necrotizing soft tissue infections. Treatment of necrotizing soft tissue infections requires the administration of appropriate antimicrobial agents and adequate surgical debridement.
View Article and Find Full Text PDFNovel biopolymer-based active packaging material for chilled beef applications: Collagen peptide-astragaloside nanocomplexes incorporated into oxidized hydroxypropyl starch/chitosan films.
Int J Biol Macromol
January 2025
College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China.. Electronic address:
The present study intended to investigate the properties of collagen peptide (CP)-astragaloside (AG) nanocomplexes (CPANs) improved oxidized hydroxypropyl starch (OHS)/chitosan (CS) (OC) film and to explore the preservation of chilled beef. The results indicated that AG significantly enhanced the stability, antioxidant capacity, and antibacterial properties of CP through mechanisms like static quenching and hydrophobic interactions. The incorporation of CPANs improved thickness, swellability, and water vapor blocking, UV-blocking and mechanical properties, antioxidant and antibacterial activity of OC film.
View Article and Find Full Text PDFRapid Preparation of Collagen/Red Blood Cell Membrane Tubes for Stenosis-Free Vascular Regeneration.
ACS Nano
January 2025
Shenzhen Key Laboratory of Smart Healthcare Engineering, Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Road, Nanshan District, Shenzhen, Guangdong 518055, PR China.
Extracellular matrix (ECM)-based small-diameter vascular grafts (SDVGs, inner diameter (ID) < 6 mm) hold great promise for clinical applications. However, existing ECM-based SDVGs suffer from limited donor availability, complex purification, high cost, and insufficient mechanical properties. SDVGs with ECM-like structure and function, and good mechanical properties were rapidly prepared by optimizing common materials and preparation, which can improve their clinical prospects.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!