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A sensitive immunoassay for the quantitation of Pig-MAP in pig saliva samples.
Res Vet Sci
November 2024
BioVetMed Research Group, Department of Animal Medicine and Surgery, Veterinary School, CEIR Campus Mare Nostrum (CMN), University of Murcia, Espinardo, 30100 Murcia, Spain. Electronic address:
The purpose of the study is to develop a sensitive assay for the proper quantification of the acute phase protein Pig-MAP in pig saliva samples. A time-resolved immunofluorometric assay (TR-IFMA) was developed using two pig-MAP-specific monoclonal antibodies. The limit of detection of the assay was 4 ng/mL, enough to measure pig-MAP concentration in saliva.
View Article and Find Full Text PDFDevelopment of an immunoassay for quantification of soluble human CD40L (CD154) in plasma and serum samples.
J Immunol Methods
August 2024
Department of Biomedicine, Aarhus University, Denmark. Electronic address:
When the membrane protein CD40 ligand (CD40L) on activated T cells binds the receptor CD40 on B-cells, it provides a co-stimulatory signal for B cell activation. Dysregulation of the CD40L:CD40 axis is associated with inflammatory and autoimmune diseases. The presence of soluble CD40L (sCD40L) in plasma is implicated in several diseases, from cardiovascular and autoimmune diseases to different types of cancer, and sCD40L has been suggested as a valuable marker of disease.
View Article and Find Full Text PDFSerum vascular adhesion protein-1 is associated with twelve-year risk of incident cancer, cancer mortality, and all-cause mortality: a community-based cohort study.
Front Oncol
December 2023
Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
Background: Vascular adhesion protein-1 (VAP-1), a dual-function glycoprotein, has been reported to play a crucial role in inflammation and tumor progression. We conducted a community-based cohort study to investigate whether serum VAP-1 could be a potential biomarker for predicting incident cancers and mortality.
Method: From 2006 to 2018, we enrolled 889 cancer-free subjects at baseline.
Platelets and the Lectin Pathway of Complement Activation in Patients with Systemic Lupus Erythematosus or Antiphospholipid Syndrome.
TH Open
April 2023
Department of Biomedicine, Aarhus University, Aarhus, Denmark.
Patients with systemic lupus erythematosus (SLE) have an increased risk of thrombosis even when they do not have antiphospholipid syndrome (APS). Interactions between complement activation and activated platelets have been suggested in SLE and APS and could play a role in the increased thrombosis risk. To explore factors potentially related to the prothrombotic pathophysiology in patients with SLE, primary APS, and healthy controls, by investigating lectin pathway proteins (LPPs), complement activation, platelet aggregation, and platelet activation.
View Article and Find Full Text PDFDiagnostic accuracy of anti-DGP (IgG) for celiac disease.
J Family Med Prim Care
January 2023
Department of Pediatric Gastroenterology, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran.
Background: Celiac disease (CD) is an autoimmune enteropathy that is caused by the intake of gluten-containing grains in genetically susceptible humans. The gliadin and glutenin parts of wheat gluten are the essential factors that cause intestinal damage.
Objectives: We analyzed the performance of a time-resolved immunofluorometric assay (TR-IFMA) in the diagnosis of CD in children.
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