AI Article Synopsis
- PI3K inhibition significantly decreases the replication of the PRRS virus by reducing viral gene transcription and protein synthesis by about 1.5-fold.
- Overexpression of a dominant negative mutant Akt1 leads to lower viral gene transcription, while the constitutively active Akt1 enhances viral protein synthesis.
- The study indicates that both PI3K and Akt1 are important in regulating viral gene expression and consequently, the replication of the virus.
Article Abstract
We have previously reported that inhibition of phosphatidylinositol 3-kinase (PI3K) reduces porcine reproductive and respiratory syndrome (PRRSV) replication. Here, we further investigate the mechanism by which PI3K inhibition affects virus replication and the role of Akt1 kinase in virus replication. We found that PI3K inhibition reduced viral gene transcription by approximately 1.5-fold. Accordingly, viral protein synthesis was significantly reduced by PI3K inhibition. Interestingly, cells overexpressing the dominant negative mutant Akt1 exhibited a significant reduction in viral gene transcription compared to cells overexpressing the constitutively active Akt1. Viral protein synthesis was also enhanced in cells overexpressing the constitutively active Akt1. Overall, our data show that both PI3K and Akt1 play a role in viral gene expression, leading to an increase in virus replication.
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| http://dx.doi.org/10.1007/s00705-014-2016-4 | DOI Listing |
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