A novel teichuronic acid, the major polymer from the cell wall of Actinoplanes lobatus VKM Ac-676(T.).

The cell wall of Actinoplanes lobatus VKM Ac-676(T) (family Micromonosporaceae; order Actinomycetales; class Actinobacteria) contains two anionic glycopolymers. The major one is a teichuronic acid with a linear structure of the chain and heterogeneous repeating units, consisting of glucopyranose residues and statistically alternating residues of diaminouronic acids having the d-manno,l-gulo and d-gluco configurations in a ratio of 6:3:1, respectively. The teichuronic acid of this structure has not hitherto been found in Gram-positive bacteria. The minor glycopolymer is a teichoic acid of poly(glycosylpolyol phosphate) nature with the repeating unit -6)-[α-d-Manp(1→4)]-β-d-Galp-(1→2)-snGro-(3-P-. The structures of the glycopolymers were established by chemical methods and NMR spectroscopy.