AI Article Synopsis
- Time-resolved ICP-MS is a technique that allows for the detailed analysis of single cells by directly introducing them into plasma for measurement, rather than analyzing bulk cell samples.
- The sensitivity and efficiency of this method depend on time resolution, which enhances cell throughput and reduces the number of cells needed for analysis.
- Using ICP-TOFMS, researchers can analyze both elements and biomolecules simultaneously, providing insights into cell-to-cell variations and improving upon traditional methods like flow cytometry.
Article Abstract
Time-resolved inductively coupled plasma mass spectrometry (ICP-MS) has attracted much attention for elemental and multiparametric analysis of single cells, instead of a classical bulk analysis of large amount of cells after a dissolution. In the time-resolved measurement, cells are directly introduced into the plasma via nebulizing or micro drop dispensing, and then ion plumes corresponding to single cells are individually detected with a high time resolution. The sensitivity and cell throughput in the measurement strongly depend on the time resolution. A high cell introduction efficiency into the plasma supports for a reduction of cell consumption. Biomolecules can also be measured through the attachment of elemental tags, and then the amount distribution of elements and biomolecules in single cells can be evaluated, while providing information concerning cell-to-cell variations. By applying ICP time-of-flight mass spectrometry (ICP-TOFMS), multiparametric analysis of elements and biomolecules can be achieved similar to that by a flow cytometer. This article highlights the technical aspects of the time-resolved ICP-MS measurement technique for elemental and multiparametric analysis of single cells.
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| http://dx.doi.org/10.2116/analsci.30.219 | DOI Listing |
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