The successful synthesis of amylose brushes via enzymatic "grafting from" polymerization and the detailed characterization of all synthetic steps by X-ray photoelectron spectroscopy (XPS) and spectroscopic ellipsometry measurements are reported. Au and Si surfaces are amino-functionalized with self-assembled monolayers (SAMs) of cystamine and 3-aminopropyldimethyethoxysilane (APDMES), respectively. Maltoheptaose is covalently attached to the amino-functionalized Au and Si surfaces via reductive amination. Amylose brushes are grown from maltoheptaose modified Au and Si surfaces with enzymatic polymerization using potato phosphorylase and Rabbit Muscle phosphorylase, as evidenced by spectroscopic ellipsometry and XPS measurements.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/mabi.201300273 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Oral intake of slowly digestible -glucan, isomaltodextrin, stimulates glucagon-like peptide-1 secretion in the small intestine of rats.
Br J Nutr
March 2020
College of Agriculture, Academic Institute, Shizuoka University, Shizuoka422-8529, Japan.
To investigate whether oral intake of highly branched α-glucan isomaltodextrin (IMD) could stimulate ileal glucagon-like peptide-1 (GLP-1) secretion, we examined (1) the digestibility of IMD, (2) the digestion and absorption rates of IMD, in rat small intestine and (3) portal GLP-1 concentration in rats given IMD. In Expt 1, ileorectostomised rats were given a 3 % IMD diet for 10 d. Separately, a 16-h in vitro digestion of IMD, using porcine pancreatic α-amylase and brush-border membrane vesicles from rat small intestine, was conducted.
View Article and Find Full Text PDFStarch digestion kinetics and mechanisms of hydrolysing enzymes in growing pigs fed processed and native cereal-based diets.
Br J Nutr
May 2019
1Animal Nutrition Group, Wageningen University and Research,De Elst 1,6708 WD Wageningen,The Netherlands.
This study aimed to examine in vivo starch digestion kinetics and to unravel the mechanisms of starch hydrolysing enzymes. Ninety pigs (23 (sd 2·1) kg body weight) were assigned to one of nine treatments in a 3×3 factorial arrangement, with starch source (barley, maize, high-amylose (HA) maize) and form (isolated, within cereal matrix, extruded) as factors. We determined starch digestion coefficients (DC), starch breakdown products and digesta retention times in four small-intestinal segments (SI1-4).
View Article and Find Full Text PDFLaboratory-Scale Preparative Enantioseparations of Pharmaceutically Relevant Compounds on Commercially Available Chiral Stationary Phases for HPLC.
Curr Med Chem
July 2017
Department of Pharmaceutical Sciences, University of Perugia, Via Fabretti 48, 06123 Perugia, Italy.
In response to the outburst of research in the field of synthetic medicinal chemistry, enantioselective chromatography methods based on the use of chiral stationary phases (CSPs) found immediate acceptance as the elective choice for the analytical determinations of the enantiomeric purity of synthetic compounds. In contrast to an initial scepticism, also the preparative-scale applications are gaining increasing recognition as a powerful alternative to enantioselective synthesis for the supply of pure enantiomers of bioactive compounds. The increasing success of liquid chromatography methods has been made possible thanks to the development of highly efficient CSPs allowing the enantioresolution of practically all the chemical classes of chiral compounds.
View Article and Find Full Text PDFEnzymatic synthesis of amylose brushes revisited: details from X-ray photoelectron spectroscopy and spectroscopic ellipsometry.
Macromol Biosci
February 2014
Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, NL-9747 AG Groningen, The Netherlands.
The successful synthesis of amylose brushes via enzymatic "grafting from" polymerization and the detailed characterization of all synthetic steps by X-ray photoelectron spectroscopy (XPS) and spectroscopic ellipsometry measurements are reported. Au and Si surfaces are amino-functionalized with self-assembled monolayers (SAMs) of cystamine and 3-aminopropyldimethyethoxysilane (APDMES), respectively. Maltoheptaose is covalently attached to the amino-functionalized Au and Si surfaces via reductive amination.
View Article and Find Full Text PDFThe dynamics of complex formation between amylose brushes on gold and fatty acids by QCM-D.
Biomacromolecules
October 2013
Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, NL-9747 AG Groningen, The Netherlands.
Amylose brushes were synthesized by enzymatic polymerization with glucose-1-phosphate as monomer and rabbit muscle phosphorylase b as catalyst on gold-covered surfaces of a quartz crystal microbalance. Fourier transform infrared (FT-IR) spectra confirmed the presence of the characteristic absorption peaks of amylose between 3100 cm(-1) and 3500 cm(-1). The thickness of the amylose brushes-measured by Spectroscopic Ellipsometry--can be tailored from 4 to 20 nm, depending on the reaction time.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!