We present a novel ultra-high resolution Raman spectroscopy technique based in a Fabry-Pérot/triple spectrometer tandem with multichannel acquisition. We describe the system, detail the calibration process, and experimentally test the technique, showing that effective finesses in excess of 1000 are possible. The technique is specifically tailored for low intensity, complex and spectrally extended Raman spectra, providing shorter acquisition times with respect to similar tandem systems with monochannel detectors.
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http://dx.doi.org/10.1063/1.4861345 | DOI Listing |
Methods Protoc
December 2024
Univ Brest, CEMCA, CNRS, UMR 6521, 29238 Brest, France.
Cyclic peptides have higher stability and better properties as therapeutic agents than their linear peptide analogues. Consequently, intramolecular click chemistry is becoming an increasingly popular method for the synthesis of cyclic peptides from their isomeric linear peptides. However, assessing the purity of these cyclic peptides by mass spectrometry is a significant challenge, as the linear and cyclic peptides have identical masses.
View Article and Find Full Text PDFSe Pu
January 2025
Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, China.
Lipids are indispensable components of living organisms and play pivotal roles in cell-membrane fluidity, energy provision, and neurotransmitter transmission and transport. Lipids can act as potential biomarkers of diseases given their abilities to indicate cell-growth status. For example, the lipid-metabolism processes of cancer cells are distinct from those of normal cells owing to their rapid proliferation and adaptation to ever-changing biological environments.
View Article and Find Full Text PDFObjective: Development and validation of a sensitive and rapid method for moxonidine detection and quantification in blood and/or urine in clinical toxicology and forensic chemistry.
Material And Methods: The method included the following stages: acetonitrile extraction, centrifugation, identification, and quantitative analysis of moxonidine in cadaveric blood and/or urine by high-performance liquid chromatography with a triple quadrupole mass spectrometer.
Results: The method was validated by the following parameters: limit of detection, limit of quantification, linearity, trueness, and precision.
J Am Soc Mass Spectrom
December 2024
School of Electronic and Information Engineering, Soochow University, Suzhou 215006, China.
Tandem mass spectrometry (MS) is one of the most effective methods to obtain the structures of organic molecules, enabling the observation of multigenerational ion fragments. Collision-induced dissociation (CID) is currently the most mature technique for mass spectrometry analysis. Ion trap mass spectrometry (ITMS) is favored for on-site detection field, due to its ability of MS analysis with a single trap and its small size.
View Article and Find Full Text PDFBiochim Biophys Acta Mol Cell Res
December 2024
Department of Anesthesiology, Faculty of Medicine, Shimane University, 89-1 Enya-cho, Izumo city, Shimane 693-8501, Japan. Electronic address:
Thrombomodulin is predominantly expressed on vascular endothelial cells and modulates endothelial cell functions by interacting with multiple ligands. The specific thrombomodulin receptor or cofactor active on the endothelial cell surface remains elusive. This study aims to identify interacting partners of thrombomodulin on endothelial cells.
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