AI Article Synopsis

  • This paper outlines a method using ultra-high-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) to identify 41 different antibiotics from seven classes in gilthead sea bream (a type of fish).
  • The researchers tested 14 sample treatment procedures and found that using a combination of acetonitrile and ethylenediaminetetra acetic acid yielded the best results for extracting antibiotics.
  • The method was validated according to EU regulations, showing high precision (RSD under 17%) and recovery rates between 92% and 111%, making it effective for routine monitoring of antibiotic residues in this fish, crucial in aquaculture.

Article Abstract

This paper describes a method for the determination of 41 antibiotics from seven different classes in gilthead sea bream (Sparus aurata) by ultra-high-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS). Sulfonamides, trimethoprim, tetracyclines, macrolides, quinolones, penicillins and chloramphenicol were simultaneously determined. Fourteen procedures for sample treatment were tested and an extraction with acetonitrile and ethylenediaminetetra acetic acid was found to be the best option. The methodology was validated in accordance with Decision 2002/657/EC. Precision in terms of relative standard deviation (RSD) was under 17% for all compounds, and the recoveries ranged from 92% to 111%. CCα and CCβ were determined according to the maximum residue limit or the minimum required performance limit, when necessary. The validation provided evidence that the method was suitable for application in routine analysis for the detection and confirmation of antibiotics in muscle of gilthead sea bream, an important and intensively produced fish in aquaculture.

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Source
http://dx.doi.org/10.1080/19440049.2014.891764DOI Listing

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