The sensitivity of filter-based fluorescence microscopy techniques is limited by autofluorescence background. Time-gated detection is a practical way to suppress autofluorescence, enabling higher contrast and improved sensitivity. In the past few years, three groups of authors have demonstrated independent approaches to build robust versions of time-gated luminescence microscopes. Three detailed, step-by-step protocols are provided here for modifying standard fluorescent microscopes to permit imaging time-gated luminescence.
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http://dx.doi.org/10.1002/0471142956.cy0222s67 | DOI Listing |
PLoS One
December 2024
Department of Electrical Engineering, Stanford University, Stanford, California, United States of America.
Small
November 2024
College of Life Science, Dalian Minzu University, Dalian, 116600, China.
The significance of HO as a marker of reactive oxygen species (ROS) and oxidative stress in living organisms has spurred growing interest in its roles in inflammation and disease progression. In this report, a ratiometric time-gated luminescence (RTGL) probe is proposed based on mixed lanthanide complexes, ER-BATTA-Tb/Eu, for imaging the HO generation both in vitro and in vivo. Upon exposure to HO, the probe undergoes cleavage of the benzyl boric acid group, releasing hydroxyl (─OH) groups, which significantly reduces the emission of the Eu complex while slightly increasing the emission of the Tb complex.
View Article and Find Full Text PDFAnal Chem
November 2024
College of Life Science, Dalian Minzu University, Dalian 116600, China.
Drug-induced liver injury (DILI) is a major hepatic dysfunction commonly caused by hepatotoxic drug overdose, resulting in a considerable number of fatalities worldwide. Recent studies have highlighted the regulatory and hepatoprotective effects of carbon monoxide (CO) during the liver injury process. However, precisely tracking the dynamic changes in the composition of CO in DILI is still a great challenge.
View Article and Find Full Text PDFNat Commun
November 2024
Beijing Key Laboratory of Energy Conversion and Storage Materials, Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing, 100875, P. R. China.
Langmuir
November 2024
Chemical Engineering, Muenster University of Applied Sciences, Stegerwaldstr. 39, Steinfurt 48565, Germany.
The ability to effectively monitor singlet oxygen (O) with fluorescence probes in biological systems is severely restricted mainly by the background autofluorescence of these systems. Though the application of lanthanide complexes as O monitors successfully resolves this problem with time-gated luminescence measurements, the insolubility of these complexes in an aqueous medium heavily limits their application in biological systems. Here, we present a water-soluble O sensor based on a chitosan-europium hybrid material.
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