AI Article Synopsis
- The study investigated the effects of cerium oxide nanoparticles (CeO2 NPs) on human neuroblastoma cells (IMR32), focusing on their cytotoxic, genotoxic, and oxidative stress responses.
- Various assays were used to measure cytotoxicity (e.g., tetrazolium and lactate dehydrogenase assays) and genotoxicity (e.g., micronucleus and comet assays), along with tests to assess oxidative stress levels.
- The findings indicated that nanosized CeO2 was more toxic than larger cerium oxide microparticles, suggesting the need for further safety evaluations of these nanoparticles in other biological models.
Article Abstract
The present study consisted of cytotoxic, genotoxic, and oxidative stress responses of human neuroblastoma cell line (IMR32) following exposure to different doses of cerium oxide nanoparticles (CeO2 NPs; nanoceria) and its microparticles (MPs) for 24 hours. Cytotoxicity was evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide and lactate dehydrogenase assays whereas genotoxicity was assessed using the cytokinesis-block micronucleus and comet assays. A battery of assays including lipid peroxidation, reactive oxygen species (ROS), hydrogen peroxide, reduced glutathione, nitric oxide, glutathione reductase, glutathione peroxidase, superoxide dismutase, catalase, and glutathione S-transferase were performed to test the hypothesis that ROS was responsible for the toxicity of nanoceria. The results showed that nanosized CeO2 was more toxic than cerium oxide MPs. Hence, further study on safety evaluation of CeO2 NPs on other models is recommended.
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| http://dx.doi.org/10.1177/1091581814522305 | DOI Listing |
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