Listeria monocytogenes: cultivation and laboratory maintenance.

Curr Protoc Microbiol

Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky.

Published: November 2013

This unit describes general procedures for the lab cultivation and storage of the Gram-positive facultative intracellular bacterium Listeria monocytogenes. The basic protocols are relevant for a wide scope of applications including microbial genetics and both in vitro and in vivo infection studies. Commonly used L. monocytogenes strains, serotypes, and growth parameters are also discussed.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3920655PMC
http://dx.doi.org/10.1002/9780471729259.mc09b02s31DOI Listing

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