Genetic diversity of Escherichia coli isolated from ducks and the environment using enterobacterial repetitive intergenic consensus.

Pak J Biol Sci

Department of Animal Science, Faculty of Agriculture, University for Development Studies, P.O. Box TL 1882, Tamale, Ghana.

Published: October 2013

Escherichia coli are mostly free living bacteria that harbour the gastrointestinal tract of poultry. Howbeit, pathogenic Escherichia coli are very important foodborne pathogens that can cause severe complications, illnesses and deaths in humans. The objective of this study was to determine the genetic diversity or relatedness of 62 Escherichia coli strains isolated from ducks and the environment using Enterobacterial Repetitive Intergenic Consensus (ERIC). The analysis of the Escherichia coli strains by ERIC produced DNA bands of different sizes for differentiation purposes and cluster analysis at a coefficient of 0.85 grouped the strains into different clusters and singletons. At this coefficient the Escherichia coli strains were grouped into thirteen clusters and eleven singletons with discriminatory index (D value) of 0.946. The ERIC PCR adapted in this study showed to be a useful genotyping tool for determining the genetic relatedness of the duck Escherichia coli strains. Comparison of the genetic relatedness among foodborne pathogens is important for foodborne diseases outbreak investigations.

Download full-text PDF

Source
http://dx.doi.org/10.3923/pjbs.2013.1173.1178DOI Listing

Publication Analysis

Top Keywords

escherichia coli
28
coli strains
16
genetic diversity
8
isolated ducks
8
ducks environment
8
environment enterobacterial
8
enterobacterial repetitive
8
repetitive intergenic
8
intergenic consensus
8
foodborne pathogens
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!