The helminth Baylisascaris schroederi is one of the most harmful parasites infecting giant pandas (Ailuropoda melanoleuca). It is therefore important to develop an exact diagnostic technique to detect this parasite. Using a known number (1, 2, 3, 4, 5, 10, 25, 50, 100) of feces-isolated B. schroederi egg and adult DNA, we developed a PCR to detect a portion of the mitochondrial 12S rRNA and applied it to giant panda fecal samples. The method was sufficiently sensitive to detect B. schroederi DNA from isolated eggs in a fecal sample with a detection threshold of one egg. We detected B. schroederi in 88% of fecal samples, 30% higher than the conventional flotation technique. No cross-reactivity with other common nematode DNA was detected. Our PCR assay may constitute a valuable alternative for the diagnosis of B. schroederi infections.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.7589/2012-07-175 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Enhanced protective immunity against Baylisascaris schroederi infection in mice through a multi-antigen cocktail vaccine approach.
Parasitol Res
December 2023
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan, China.
Baylisascaris schroederi is among the most severe intestinal nematodes affecting giant pandas. Developing effective and secure vaccines can be used as a novel strategy for controlling repeated roundworm infection and addressing drug resistance. In our previous study, three recombinant antigens (rBsHP2, rBsGAL, and rBsUP) exhibited promising effects against B.
View Article and Find Full Text PDFEvaluation of the immunoprotective effects of eight recombinant proteins from Baylisascaris schroederi in mice model.
Parasit Vectors
July 2023
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China.
Article Synopsis
- Baylisascaris schroederi is a harmful parasitic worm affecting giant pandas, leading to health issues like ascariasis, and current treatments may lead to drug resistance.
- Researchers are exploring a vaccine by using specific proteins from the parasite to stimulate an immune response in mice and reduce future infections.
- Vaccination with certain proteins significantly decreased larval worms in mice and improved their health, indicating potential for developing an effective vaccine for giant pandas.
Investigation of the Efficacy of Pyrantel Pamoate, Mebendazole, Albendazole, and Ivermectin against in Captive Giant Pandas.
Animals (Basel)
December 2022
College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China.
is one of the main health risks threatening both wild and captive giant pandas. The administration of anthelmintics is a common method to effectively control infection, but there is a notable risk of anthelmintic resistance (AR) after long-term, constant use of anthelmintics. Four anthelmintics-pyrantel pamoate (PYR), mebendazole (MBZ), albendazole (ABZ), and ivermectin (IVM)-were each administered separately at intervals of 2 months to 22 enrolled giant pandas.
View Article and Find Full Text PDFA novel cysteine protease inhibitor in Baylisascaris schroederi migratory larvae regulates inflammasome activation through the TLR4-ROS-NLRP3 pathway.
Parasit Vectors
September 2022
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 611130, China.
Background: Giant pandas (Ailuropoda melanoleuca) are the obligate host of the parasitic roundworm Baylisascaris schroederi. The infection of giant pandas with B. schroederi is very common.
View Article and Find Full Text PDFCharacterization of a novel cysteine protease inhibitor in migratory larvae and its role in regulating mice immune cell response.
Front Immunol
March 2023
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China.
() is a severe threat to the survival of giant pandas. Currently, the immune regulation mechanism of is poorly understood. Cysteine protease inhibitors (CPI) play important roles in the regulation of host immune responses against certain nematodes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!