The garlic polysaccharide was modified by HNO3-Na2SeO3 method according to orthogonal design L9(3(4)) to obtain nine selenizing garlic polysaccharides, sGPS1-sGPS9. Their effects on chicken peripheral lymphocytes proliferation in vitro were compared by MTT assay. The results showed that sGPSs could significantly promote lymphocytes proliferation, sGPS3, sGPS5 and sGPS6 presented stronger efficacy. In vivo experiment, 14-day-old chickens were injected respectively with sGPS3, sGPS5 and sGPS6 when they were vaccinated with ND vaccine taking unmodified GPS as control. The results showed that three sGPSs could significantly promote lymphocyte proliferation, enhance serum antibody titer, IFN-γ and IL-2 contents. These results indicated that selenylation modification could significantly enhance the immune-enhancing activity of GPS, sGPS6 possessed the best efficacy and could be as a candidate drug of immunoenhancer. Its optimal modification conditions were 400 mg of sodium selenite for 500 mg of GPS, reaction temperature of 70°C and reaction time of 6 h.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907442 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0086377 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
Modification of a O-acetyl-glucomannan from Dendrobium officinale by selenylation modification and its anti-gastric cancer enhancing activity.
Int J Biol Macromol
December 2024
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, People's Republic of China. Electronic address:
In this study, a homogeneous polysaccharide, named YDOP-1 was isolated form Dendrobium officinale using hot water extraction and ethanol precipitation method. YDOP-1 was characterized to be a typical O-acetyl-glucomannan with the molecular wight was 13,456 Da. Cell viability and colony forming assay showed that YDOP-1 possess moderate anti-gastric cancer effects.
View Article and Find Full Text PDFPhysicochemical properties, structural characterization, and antidiabetic activity of selenylated low molecular weight apple pectin in HFD/STZ-induced type 2 diabetic mice.
Carbohydr Polym
January 2025
National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, No.36 Huanhu Middle Road, Jinyinhu District, Wuhan 430023, PR China; Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, No.36 Huanhu Middle Road, Jinyinhu District, Wuhan 430023, PR China. Electronic address:
A novel selenylated low molecular weight apple pectin (Se-LMWAP) was prepared through enzymatic modification combined with selenylation. The physicochemical properties, the structural characterization of Se-LMWAP were evaluated by FT-IR, NMR and SEM. Moreover, the antidiabetic activity and potential mechanism of Se-LMWAP were investigated using high-fat diet (HFD)/streptozotocin (STZ)-induced type 2 diabetic mice.
View Article and Find Full Text PDFAnalysis of Microheterogeneous Glutelin Subunits and Highly Efficient Identification of Selenylation Peptides by In-Gel Proteolysis: Focus on Se-Enriched Rice.
J Agric Food Chem
November 2024
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Article Synopsis
- Selenylation of cysteine and methionine thiols, achieved through selenate supplements, boosts selenium-containing amino acids in crops, offering various health benefits.
- The study developed a mass spectrometry technique to effectively identify selenylation peptides, discovering 11 rice glutelin subunits and multiple selenylation peptides that demonstrated high confidence in their identification.
- The findings highlight numerous selenation sites in rice glutelin subunits, suggesting that these selenylated peptides may hold potential for various health-related bioactivities, such as neuroprotection and antioxidant effects.
CuI-Catalyzed Selenylation of Pyrrolo[2,1-]isoquinolines and Other Electron-Rich Heteroarenes.
J Org Chem
October 2024
Laboratory of Asymmetric Synthesis, College of Chemistry and Environmental Engineering, Chongqing University of Arts and Sciences, 319 Honghe Ave., Yongchuan, Chongqing 402160, P.R. China.
We have established a mild CuI-catalyzed selenylation of pyrrolo[2,1-]isoquinoline derivatives in the presence of CPBA (-chloroperoxybenzoic acid) at ambient temperature. Corresponding organoselenides have been prepared readily in 53-92% yields. This process can also be expanded to the modification of pyrroles, azaindole, and indoles, delivering the desired heterocyclic selenides in moderate to good yields.
View Article and Find Full Text PDFC3 Selective chalcogenation and fluorination of pyridine using classic Zincke imine intermediates.
Nat Commun
August 2024
Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan, 610064, PR China.
Article Synopsis
- Regioselective C-H functionalization of pyridines is difficult due to their electronic properties, but this study presents a strategy for selective modifications at the C3 position using N-2,4-dinitrophenyl Zincke imine intermediates under mild conditions.
- The research involves radical inhibition and DFT calculations, showing thiolation and selenylation occur through a radical pathway, while fluorination follows a two-electron substitution mechanism.
- The study highlights the importance of the electron-deficient N-DNP group for enhancing the reaction's efficiency and also demonstrates the method's effectiveness in large-scale synthesis and modifying pharmaceutical compounds.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!