A number of additives have been tested for their effects on o-diphenol: O2 oxidoreductase activity of cane leaves. The most inhibitory compounds were thioglycollate, β-mercaptoethanol, polyethylene glycol and bovine serum albumin. Sulphydryl (SH) compounds did not affect rates of photosynthetic CO2 assimilation when used at concentrations below 10(-2) M. However, in the presence of Mn(++) ions they contributed to an O2 consumption which masked photosynthetic O2 evolution. Addition of SH compounds or of polymers to the grinding media increased rates of enzymic CO2 assimilation in crude enzyme preparations from cane leaves, but did not affect rates of CO2 assimilation in similar spinach preparations. Strong reducing agents, copper chelators, low O2 tension and high pH were effective in reducing phenoloxidase activity, but presented problems in the isolation and assay of chloroplasts. The results are discussed in relation to (a) design of suitable media for preparation of active cane chloroplasts and (b) comparative studies of enzyme levels in plants of various genera.
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