AI Article Synopsis
- Liposomes with magnetic gold nanoparticles were developed as smart containers for detecting alkaline phosphatase (ALP) via flow injection techniques.
- The method achieved a dynamic detection range of 6.4 × 10(-3) to 0.25 U L(-1) for ALP, with a low detection limit of 1.9 × 10(-3) U L(-1) and high precision (RSD% between 0.7-2.4%).
- This technique was successfully tested on milk samples, showing recovery rates from 87.5 to 104.6% and allowing analysis of ALP activity after different temperature treatments, with results compared to previous methods.
Article Abstract
Liposomes containing magnetic gold nanoparticles (AuNPs) and an enzymatic substrate (4-methylumbelliferyl-phosphate) have been used as on-flow microcontainers for reagent preconcentration in a flow injection method for the determination of alkaline phosphatase (ALP) activity. The dynamic range of the calibration graph was 6.4 × 10(-3)-0.25 U L(-1) ALP, and the detection limit was 1.9 × 10(-3) U L(-1). The precision, expressed as relative standard deviation (RSD%), was in the range of 0.7-2.4%. The overall method showed a sampling frequency of 10 h(-1). The method was applied to the determination of ALP in milk samples with recovery values ranging between 87.5 and 104.6%. The residual ALP activity in milk samples subjected to temperature treatments was also determined. The results obtained in the analysis of all milk samples were compared with those obtained by applying a previously described flow injection method.
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| http://dx.doi.org/10.1021/jf5004804 | DOI Listing |
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