Conflict Of Interest: none declared.
Introduction: This study was carried out to evaluate PCR-based method for detection of DNA in goat milk. It utilized primers targeting the mitochondrial cytochrome -b (mtcyt-b) gene, which was used as a target DNA for PCR amplification.
Methods: For the specific identification of goat mtcyt-b gene, pair of primers (GSL1, GSR2), were used, which produced a 428 base pair (bp) PCR product from milk samples as well as from peripheral blood. Amplification products were visualized on ethidium bromide-stained agarose gels.
Results And Discussion: Amplification products were not detected when the PCR was applied to DNA from animal species including cattle, sheep, swine, camel, deer, horse, donkey, and human, which indicates that the 2 pairs of primers are specific for goat.
Conclusion: DNA can be extracted from goat milk and would be advantageous in the variety of application such as species identification in milk and milk.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769079 | PMC |
| http://dx.doi.org/10.5455/msm.2013.25.105-108 | DOI Listing |
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