(1)H, (15)N and (13)C resonance assignments of the yeast Pih1 and Tah1 C-terminal domains complex.

Biomol NMR Assign

Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), UMR 7365 CNRS, Université de Lorraine, Biopôle de l'Université de Lorraine, Campus Biologie Santé, 9 avenue de la forêt de Haye, CS 50184, 54505, Vandœuvre-lès-Nancy, France.

Published: April 2015

We report the nearly complete (1)H, (15)N and (13)C resonance assignment of the complex formed by the C-terminal domains of Pih1 and Tah1 from S. cerevisiae and evidence the folding ability of Tah1 under complex formation.

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12104-014-9547-1DOI Listing

Publication Analysis

Top Keywords

15n 13c
8
13c resonance
8
pih1 tah1
8
c-terminal domains
8
resonance assignments
4
assignments yeast
4
yeast pih1
4
tah1 c-terminal
4
domains complex
4
complex report
4

Similar Publications

Underlying Mechanisms of Chromatographic H/D, H/F, and Isomerism Effects in GC-MS.

Metabolites

January 2025

Institute of Toxicology, Core Unit Proteomics, Hannover Medical School, 30623 Hannover, Germany.

Charge-free gaseous molecules labeled with deuterium H (D) atoms elute earlier than their protium-analogs H (H) from most stationary GC phases. This effect is known as the chromatographic H/D isotope effect (IE) and can be calculated by dividing the retention times () of the protiated ( ) to those of the deuterated () analytes: IE = /. Analytes labeled with C, N or O have almost identical retention times and lack a chromatographic isotope effect.

View Article and Find Full Text PDF

Using NMR Spectroscopy to Evaluate Metal-Ligand Bond Covalency for the f Elements.

Acc Chem Res

January 2025

Department of Chemistry, University at Buffalo, State University of New York, Buffalo, New York 14260, United States.

ConspectusUnderstanding f element-ligand covalency is at the center of efforts to design new separations schemes for spent nuclear fuel, and is therefore of signficant fundamental and practical importance. Considerable effort has been invested into quantifying covalency in f element-ligand bonding. Over the past decade, numerous studies have employed a variety of techniques to study covalency, including XANES, EPR, and optical spectroscopies, as well as X-ray crystallography.

View Article and Find Full Text PDF

Gas-phase conformational landscape and ring-puckered structure of 1-aminoindane.

Chemphyschem

January 2025

Universidad de Valladolid Facultad de Ciencias, Química Física y Química Inorgánica, SPAIN.

Indane-based molecules are effective scaffolds for different pharmaceutical products, so it is relevant to analyze the relation between structure and functionality in indane derivatives. Here, we have characterized the conformational landscape and molecular structure of 1-aminoindane in the gas phase using chirped-excitation Fourier-transform microwave spectroscopy and computational methods. The rotational spectrum confirmed the presence of two conformers, which were identified based on their rotational constants and 14N nuclear quadrupole coupling tensor elements.

View Article and Find Full Text PDF

Assignment of the N-terminal domain of mouse cGAS.

Biomol NMR Assign

January 2025

Department of Chemistry and Chemical Biology, TU Dortmund University, Dortmund, Germany.

Cyclic GMP-AMP synthase (cGAS) is a DNA-sensing enzyme that is a member of the nucleotidyltransferase (NTase) family and functions as a DNA sensor. The protein is comprised of a catalytic NTase core domain and an unstructured hypervariable N-terminal domain (NTD) that was reported to increase protein activity by providing an additional DNA-binding surface. We report nearly complete H, N, and C backbone chemical-shift assignments of mouse cGAS NTD (residues 5-146), obtained with a set of 3D and 4D solution NMR experiments.

View Article and Find Full Text PDF

Solution NMR studies of large systems are hampered by rapid signal decay. We hereby introduce ROCSY (relaxation-optimized total correlation spectroscopy), which maximizes transfer efficiency across J-coupling-connected spin networks by minimizing the amount of time magnetization spends in the transverse plane. Hard pulses are substituted into the Clean-CITY TOCSY pulse element first developed by Ernst and co-workers, allowing for longer delays in which magnetization is aligned along the z-axis.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!