AI Article Synopsis
- - Vesicular trafficking is essential for regulating Notch signaling, crucial for cell fate and development of sensory organs.
- - The gene tempura encodes a protein that forms a novel complex, which modifies Rab proteins (Rab1 and Rab11) to ensure their proper location within the cell.
- - Impairments in Rab1 and Rab11 due to tempura loss disrupt the secretion of Scabrous and Delta, leading to issues in Notch signaling and sensory organ development.
Article Abstract
Vesicular trafficking plays a key role in tuning the activity of Notch signaling. Here, we describe a novel and conserved Rab geranylgeranyltransferase (RabGGT)-α-like subunit that is required for Notch signaling-mediated lateral inhibition and cell fate determination of external sensory organs. This protein is encoded by tempura, and its loss affects the secretion of Scabrous and Delta, two proteins required for proper Notch signaling. We show that Tempura forms a heretofore uncharacterized RabGGT complex that geranylgeranylates Rab1 and Rab11. This geranylgeranylation is required for their proper subcellular localization. A partial dysfunction of Rab1 affects Scabrous and Delta in the secretory pathway. In addition, a partial loss Rab11 affects trafficking of Delta. In summary, Tempura functions as a new geranylgeranyltransferase that regulates the subcellular localization of Rab1 and Rab11, which in turn regulate trafficking of Scabrous and Delta, thereby affecting Notch signaling.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3904817 | PMC |
| http://dx.doi.org/10.1371/journal.pbio.1001777 | DOI Listing |
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