In this study, an ultra-sensitive method for the quantification of lysozyme based on the Gd(3+) diethylenetriamine-N,N,N',N″,N″-pentaacetic acid labeling and capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP-MS) was described. The Gd(3+)-tagged lysozyme was effectively separated by capillary electrophoresis (CE) and sensitively determined by inductively coupled plasma mass spectrometry (ICP-MS). Based on the gadolinium-tagging and CE-ICP-MS, the lysozyme was determined within 12 min with an extremely low detection limit of 3.89 attomole (3.89×10(-11) mol L(-1) for 100 nL of sample injection) and a RSD<6% (n=5). The proposed method has been successfully used to detect lysozyme in saliva samples with a recovery of 91-106%, suggesting that our method is sensitive and reliable. The success of the present method provides a new potential for the biological assays and sensitive detection of low-abundant proteins.
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http://dx.doi.org/10.1016/j.aca.2014.01.003 | DOI Listing |
Pharmaceutics
November 2024
Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
This study aimed to develop a quantitative analytical method for the simultaneous determination of cannabidiol (CBD) and melatonin (MT) in mouse plasma using the protein precipitation method coupled with LC-MS/MS. Additionally, this study sought to investigate the impact of CBD on the pharmacokinetics of MT in mice using this method. Mouse plasma samples were precipitated with acetonitrile and analyzed using a Kromasil 100-5-C8 (2.
View Article and Find Full Text PDFPlants (Basel)
December 2024
Xinjiang Key Laboratory for Ecological Adaptation and Evolution of Extreme Environment Biology, College of Life Sciences, Xinjiang Agricultural University, Urumqi 830052, China.
Plant plasma membrane (PM) H-ATPase functions as a proton-motive force by exporting cellular protons to establish a transmembrane chemical gradient of H ions and an accompanying electrical gradient. These gradients are crucial for plant growth and development and for plant responses to abiotic and biotic stresses. In this study, a comprehensive identification of the PM H-ATPase gene family was conducted across four cotton species.
View Article and Find Full Text PDFToxics
December 2024
Environmental, Genetics, and Nutritional Epidemiology Research Center (CREAGEN), Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, 41125 Modena, Italy.
Background: A limited number of studies have investigated the role of environmental chemicals in the etiology of mild cognitive impairment (MCI). We performed a cross-sectional study of the association between exposure to selected trace elements and the biomarkers of cognitive decline.
Methods: During 2019-2021, we recruited 128 newly diagnosed patients with MCI from two Neurology Clinics in Northern Italy, i.
Toxics
December 2024
Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, and Institute of Marine Sciences, Shantou University, Shantou 515063, China.
With the rapid industrialization and urbanization of coastal areas, marine pollution (such as heavy metals) is increasingly contaminating the environment, posing significant public health risks. Eastern Guangdong, a key aquaculture and fisheries hub in China, has a growing market for aquatic products. Heavy metals persist in the environment and are difficult to degrade and bioaccumulate in marine organisms through the food web, presenting carcinogenic and mutagenic risks to humans, as top predators.
View Article and Find Full Text PDFToxics
November 2024
Medical School, Southeast University, Nanjing 210096, China.
: Limited evidence links urinary metal exposure to osteoporosis in broad populations, prompting this study to cover this knowledge gap using supervised and unsupervised approaches. : This study included 15,923 participants from the National Health and Nutrition Examination Survey (NHANES) spanning from 1999 to 2020. Urinary concentrations of nine metals-barium (Ba), cadmium (Cd), cobalt (Co), cesium (Cs), molybdenum (Mo), lead (Pb), antimony (Sb), thallium (Tl), and tungsten (Tu)-were measured using inductively coupled plasma mass spectrometry (ICP-MS).
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!