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Detecting changes of testicular interstitial cell membranes with a fluorescent probe after incubation and cryopreservation with cryoprotective agents.
Cryobiology
January 2025
The National Technical University "Kharkiv Polytechnic Institute", 2 Kyrpychova st, 61000, University, 6 Trinklera st, 61022, Kharkiv, Ukraine; Research Institute of Experimental and Clinical Medicine, Kharkiv National Medical University, 6 Trinklera st, 61022 Kharkiv, Ukraine. Electronic address:
Membrane alterations are among central factors predetermining cell survival during cryopreservation. In the present research, we tested some serum-/xeno-free cryoprotective compositions including dimethyl sulfoxide (MeSO) and polymers for their osmotic impact and toxicity towards testicular interstitial cells (ICs). IC survival was determined after their contact with MeSO, dextran (D40), hydroxyethyl starch (HES), polyethylene glycols (PEG1500 and PEG400), or after cryopreservation and cryoprotective agent (CPA) removal.
View Article and Find Full Text PDFWhen Elevated Creatinine Is Not What It Seems: Intraperitoneal Urinary Leak Mimicking Acute Kidney Injury.
Cureus
December 2024
Hospital Medicine, Cleveland Clinic Abu Dhabi, Abu Dhabi, ARE.
Acute kidney injury (AKI) is typically classified as prerenal, renal, or postrenal in etiology, with postrenal often referring to obstructive causes. However, certain uncommon conditions, such as intraperitoneal urinary leaks, may not fit clearly into these categories. In patients with a recent history of pelvic procedure, a complication such as intraperitoneal urinary leak can mimic AKI due to urine reabsorption across the peritoneum.
View Article and Find Full Text PDFSalivary pH, but not conductivity, is an indicator of diarrhea in neonatal calves.
Front Vet Sci
December 2024
Scotland's Rural College (SRUC), Edinburgh, United Kingdom.
Article Synopsis
- Neonatal calf diarrhea frequently leads to dehydration and metabolic acidosis, impacting calf mortality, growth, and productivity, prompting interest in technological disease detection.
- The study explored the relationship between saliva conductivity and pH with clinical and biochemical indicators in calves, assessing the health of 141 dairy-bred calves from one week old until 25 days or until they developed diarrhea.
- Results indicated that while saliva pH decreased in calves with diarrhea, saliva conductivity showed no correlation with diarrhea or dehydration, highlighting the complexity of hydration status assessments in these animals.
The Molecular Detection and Therapeutic Management of Pathogenic Infection in a Goat: A Case Report.
Iran J Parasitol
January 2024
Department of Veterinary Medicine, College of Veterinary Science, Assam, India.
A 2-year-old female Assam Hill goat was presented with a clinical history of anorexia, fever, mild anemia, rough body coat, dehydration, tachycardia, dyspnea and swelling of palpable lymph nodes. Hematology revealed low hemoglobin, packed cell volume, red blood cell and thrombocyte count. Biochemical analysis showed increased serum concentration of alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine and urea in comparison to the normal reference range.
View Article and Find Full Text PDFCorrelation of Red Blood Cell Morphology with Serum Cobalamin and Folate Concentration in Dogs with Chronic Diarrhea: A Retrospective Study.
Metabolites
November 2024
Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54643 Thessaloniki, Greece.
: Chronic diarrhea in dogs is a prevalent condition that significantly impacts canine health, often leading to weight loss, dehydration, and malnutrition. Diagnosing and treating chronic diarrhea is challenging due to its multifactorial nature, necessitating collaboration among veterinarians across various specialties. Measuring cobalamin and folate levels is a crucial diagnostic step for all dogs with chronic diarrhea.
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