Protein splicing technology harnesses the ability of inteins to ligate protein fragments, forming a mature protein. This report describes our effort to engineer rapamycin-dependent protein splicing of a ribotoxin, called α-sarcin. Engineering this system required the investigation of important splicing parameters, including extein context and splicing temperature. We show α-sarcin splicing is dependent on rapamycin, is inducible with rapid kinetics, and triggers apoptosis in HeLa cells. These findings establish a proof-of-concept for a conditional cell ablation strategy.
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| http://dx.doi.org/10.1039/c3mb70387h | DOI Listing |
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Institute of Health Sciences, Department of Medical and Surgical Research, Hacettepe University, Ankara, Turkey.
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Euroasian J Hepatogastroenterol
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