AI Article Synopsis
- A study cloned and analyzed the 5'-flanking region of the uteroglobin (UG) gene from the brown hare and compared it to similar genes in rabbits and volcano rabbits.
- The UG gene is highly conserved among these species, sharing essential genetic elements, including a functional estrogen response element (ERE), which is crucial for understanding estrogen regulation.
- Notably, the brown hare's ERE closely matches that of rabbits, displaying some differences with the volcano rabbit, and demonstrates species-specific variations in estrogen-regulated transcription efficiency.
Article Abstract
To get further insights on the estrogen regulation of the uteroglobin (UG) gene, the 5'-flanking region of the UG gene from the brown hare (Lepus capensis) (Lc) was cloned and compared with those from two phylogenetically related species: the rabbit (Orictolagus cuniculus) (Oc) and the volcano rabbit (Romerolagus diazi) (Rd). The Lc-UG gene is very similar to those from rabbits (94%) and volcano rabbits (95%), and shares a number of genetic elements, including an estrogen response element (ERE). The estrogen-regulated transcription of a series of progressive 5'-deletion mutants of the Lc-UG gene, identified a functional ERE in the promoter region exhibiting the same orientation and relative position than that previously described in rabbits. The Lc-ERE is identical to the Oc-ERE, but different from both the Rd-ERE and the consensus ERE (c-ERE) by one nucleotide. We also detected important species-specific differences in the estrogen-regulated transcription of the UG gene. A luciferase reporter driven by 333 base pairs (bp) of the Lc-UG promoter elicited a higher response to estradiol than its related counterparts when expressed in estrogen-sensitive MCF-7 cells. Several ERE-like motifs which failed to act as functional EREs were also identified; one of them exhibited two mismatches in its palindromic sequence, a characteristic exhibited in many other natural occurring EREs, including the Rd-ERE.
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| http://dx.doi.org/10.1016/j.ygcen.2014.01.006 | DOI Listing |
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