AI Article Synopsis
- Curcuminoids, which include curcumin, demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC), offer clinical benefits for chronic diseases like osteoarthritis and type II diabetes, but their low oral bioavailability poses challenges.
- A novel liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been developed to simultaneously quantify curcuminoids and their metabolites in human plasma.
- This method demonstrates good accuracy and precision in measuring these compounds, and it has been validated for use in pharmacokinetic studies following an oral dose of bioavailable curcumin.
Article Abstract
Curcuminoids, a mixture of curcumin, demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC), have shown a variety of clinical benefits for several human chronic diseases including osteoarthritis, rheumatoarthritis, and type II diabetes. However, the oral bioavailability of curcumin is extremely low due to its avid metabolism to curcumin O-glucuronide (COG), curcumin O-sulfate (COS), tetrahydrocurcumin (THC), and other minor metabolites. This paper reports a unique liquid chromatography/tandem mass spectrometry (LC-MS/MS) method to quantify curcumin, DMC, BDMC, COG, COS, and THC simultaneously in human plasma. These compounds were extracted with ethyl acetate from human plasma, separated on a BetaBasic-8 column, and monitored on a triple quadruple mass spectrometer coupled with API electrospray under a negative ion mode. The linearity of these respective curcuminoids and curcumin metabolites was shown in the range of 2-1000ng/mL with 85-115% accuracy and ≤20% precision in human plasma. This method was validated according to the US FDA GLP analytic criteria and applied to characterize the pharmacokinetics of curcumin, COG, and COS in human plasma after an oral dose of bioavailable curcumin (nanoemulsion curcumin).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4188440 | PMC |
| http://dx.doi.org/10.1016/j.jchromb.2013.12.039 | DOI Listing |
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