It has been suggested that the intra-erythrocyte levels of methotrexate (MTX) and its polyglutamized derivatives could offer a method for evaluating intracellular MTX accumulation and its metabolism in children with acute lymphoblastic leukemia. We were interested in measuring the intra-erythrocyte levels of MTX in patients with solid tumors receiving high-dose MTX treatment. After a first incorporation stage occurring during infusion, MTX concentrations subsequently increased 9-12 days after the treatment as polyglutamized derivatives. Thirty days after the infusion, MTX and its polyglutamates were still measurable in erythrocytes. The percentage of polyglutamization varied on an individual basis, but two groups of patients could be separated according to their ability to form polyglutamates. We also noted the presence of 7-hydroxy-methotrexate (7-OH-MTX) appearing 48 hours after the beginning of the infusion which was still present in 17/20 samples 30 days after the treatment.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1089/cdd.1987.4.119 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effect of lycopene supplementation on lipid profile, blood glucose and electrolyte homeostasis in thioacetamide induced liver cirrhosis.
Pak J Pharm Sci
July 2023
Clinical Biochemistry and Hematology Research Lab., Department of Biochemistry, Federal Urdu University of Arts, Science and Technology, Karachi, Pakistan.
Lycopene is a fat-soluble carotenoid pigment that gives tomatoes their red color and capacity for scavenging free radicals. The current study was designed to evaluate the effect of lycopenesupplementation on blood glucose, lipid profile and electrolyte homeostasis in thioacetamide induced liver cirrhosis. Experimental period was consisted of 12 weeks, divided into two phases (each of six weeks).
View Article and Find Full Text PDFEffect of Obstructive Sleep Apnea and CPAP Treatment on the Bioavailability of Erythrocyte and Plasma Nitric Oxide.
Int J Environ Res Public Health
November 2022
Clinical Department of Internal and Occupational Diseases, Hypertension and Clinical Oncology, Faculty of Medicine, Wroclaw Medical University, 50-367 Wroclaw, Poland.
Introduction: Endothelial dysfunction resulting from decreased nitric oxide (NO) bioavailability is an important mechanism that increases cardiovascular risk in subjects with obstructive sleep apnea (OSA). NO is produced by nitric oxide synthase (NOS) in a reaction that converts L-arginine to L-citrulline. Asymmetric-dimethylarginine (ADMA) is created by L-arginine and is a naturally occurring competitive inhibitor of nitric oxide synthase (NOS).
View Article and Find Full Text PDFBreathing Abnormalities During Sleep and Wakefulness in Rett Syndrome: Clinical Relevance and Paradoxical Relationship With Circulating Pro-oxidant Markers.
Front Neurol
March 2022
Rett Syndrome Trial Center, Child Neuropsychiatry Unit, University Hospital Azienda Ospedaliera Universitaria Senese, Siena, Italy.
Background: Breathing abnormalities are common in Rett syndrome (RTT), a pervasive neurodevelopmental disorder almost exclusively affecting females. RTT is linked to mutations in the methyl-CpG-binding protein 2 () gene. Our aim was to assess the clinical relevance of apneas during sleep-wakefulness cycle in a population with RTT and the possible impact of apneas on circulating oxidative stress markers.
View Article and Find Full Text PDFSalicylic acid attenuates gentamicin-induced nephrotoxicity in rabbits.
Pak J Pharm Sci
January 2021
Department of Biochemistry, Federal Urdu University of Arts, Science and Technology, Karachi, Pakistan.
Gentamicin (GM) is a generally utilized as an antibiotic against dangerous and life threatening contaminations, yet its value is restricted by the development of nephrotoxicity. The present investigation was intended to decide the defensive impact of salicylic acid (SA) in gentamicin-induced nephrotoxicity in rabbits. Quantitative assessment of gentamicin-induced structural changes and level of functional modifications in the kidneys were performed by biochemical examinations keeping in mind the end goal is to decide the potential protective impacts of SA co-administration with gentamicin.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!