We demonstrate the evolution of the PylRS/tRNA(CUA) pair for genetically encoding photocaged cysteine. By characterizing the incorporation in Escherichia coli and mammalian cells, and the photodeprotection process in vitro and in mammalian cells, we establish conditions for rapid efficient photodeprotection to reveal native proteins in live cells. We demonstrate the utility of this approach by rapidly activating TEV protease following illumination of single cells.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333589 | PMC |
| http://dx.doi.org/10.1021/ja412191m | DOI Listing |
Publication Analysis
Top Keywords
mammalian cells
12
encoding photocaged
8
photocaged cysteine
8
tev protease
8
cells demonstrate
8
cells
5
genetic encoding
4
cysteine allows
4
allows photoactivation
4
photoactivation tev
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!