AI Article Synopsis
- Low-level viremia (low viral load) during HIV treatment is becoming crucial to measure accurately, as highlighted by a study of over 4,200 paired results from four different blood tests.
- The correlation between the different assays was high (0.90 to 0.97), but significantly lower (0.45 to 0.85) at low viral loads.
- The study found that clearer guidelines are necessary to understand the clinical importance of low-level viremia, especially since results vary more at low viral load thresholds.
Article Abstract
Low-level viremia during antiretroviral therapy and its accurate measurement are increasingly relevant. Here, we present an international collaboration of 4,221 paired blood plasma viral load (pVL) results from four commercial assays, emphasizing the data with low pVL. The assays compared were the Abbott RealTime assay, the Roche Amplicor assay, and the Roche TaqMan version 1 and version 2 assays. The correlation between the assays was 0.90 to 0.97. However, at a low pVL, the correlation fell to 0.45 to 0.85. The observed interassay concordance was higher when detectability was defined as 200 copies/ml than when it was defined as 50 copies/ml. A pVL of ∼100 to 125 copies/ml by the TaqMan version 1 and version 2 assays corresponded best to a 50-copies/ml threshold with the Amplicor assay. Correlation and concordance between the viral load assays were lower at a low pVL. Clear guidelines are needed on the clinical significance of low-level viremia.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3911321 | PMC |
| http://dx.doi.org/10.1128/JCM.02461-13 | DOI Listing |
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