A mixed precipitation in the gel (MPG) technique is suggested for detection and characterization of monoclonal antibodies (MAbs). The MPG is based on the formation of a mixed precipitate composed of an antigen, the corresponding MAb and precipitating polyclonal antiserum. MAb incorporated into the precipitate is revealed by Fab'-peroxidase conjugate added to polyclonal antiserum. The MPG technique was applied to hybridoma screening as well as for the antigen and epitope specificity analysis of different MAbs. The MPG is a one-step, simple, inexpensive technique and valuable for the study of any antigen which could be revealed by immunodiffusion.
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http://dx.doi.org/10.1016/0161-5890(87)90157-x | DOI Listing |
ACS Omega
January 2025
Department of Applied Chemistry, National Yang Ming Chiao Tung University, Hsinchu 30050, Taiwan, ROC.
Here, we report the design, synthesis, and comprehensive characterization of the bis-cholesterol supramolecular gelator, which contains photochromic stiff-stilbene as a bridging unit. The -isomer of stiff-stilbene bridged bis-cholesterol (-) was first synthesized with a systematic design, which can be further converted into its -isomer (-) with a high degree of efficiency (ca. 100%) upon exposure to 385 nm UV light.
View Article and Find Full Text PDFJ Environ Manage
January 2025
The Forestry and Wetland Protection Service Centre of Panjin, Panjin Forestry and Wetland Bureau, Panjin, 124000, China. Electronic address:
The Liaohe River Estuary (LRE) wetland is a critical stopover on the East Asian-Australasian Flyway (EAAF), vital to coastal ecological balance and biodiversity. However, the drivers of changes in waterbird diversity remain unclear. This study utilised random forests to produce reliable time-series wetland mapping from 2010 to 2023.
View Article and Find Full Text PDFBMC Chem
January 2025
Environmental Applications of Nanomaterial's Lab., Department of Chemistry, Faculty of Science, Aswan University, Aswan, 81528, Egypt.
Water is one of the vital needs of life. However, due to rapid industrialization, urbanization and lack of awareness, the world population now facing the threat of water shortage. To ensure that future living conditions are preserved, it is crucial to reduce water pollution and protect the ecosystem.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou 434023, People's Republic of China; China National Petroleum Corporation HSE Key Laboratory (Yangtze University Research Laboratory), Jingzhou 434023, People's Republic of China.
The incorporation of ZIF-67 into hydrogels for wastewater pollutant remediation has been widely studied, but the synthesis often requires organic solvents such as methanol or ethanol, which can result in the generation of toxic liquid waste. In this study, a novel hydrogel (ZIF-67@SL) was synthesized by integrating ZIF-67 into a dual-network system of sodium lignosulfonate (SL) and acrylamide (AM) using an in situ precipitation method in water. The material was characterized by XRD, FTIR, XPS, SEM, TEM, BET, and TGA analyses.
View Article and Find Full Text PDFSci Total Environ
January 2025
School of the Environment, University of Queensland, QLD, Australia.
The transition to net zero emissions requires the capture of carbon dioxide from industrial point sources, and direct air capture (DAC) from the atmosphere for geological storage. Dissolved CO has reactivity to rock core, and while the majority of previous studies have concentrated on reservoir rock or cap-rock reactivity, the underlying seal formation may also react with CO. Drill core from the underlying seal of a target CO storage site was reacted at in situ conditions with pure CO, and compared with an impure CO stream with SO, NO and O that could be expected from hard to abate industries.
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