Mixed precipitation in gel: a new method of identification and characterization of monoclonal antibodies.

Mol Immunol

Laboratory of Immunochemistry, Academy of Medical Sciences of the U.S.S.R., Moscow.

Published: November 1987

A mixed precipitation in the gel (MPG) technique is suggested for detection and characterization of monoclonal antibodies (MAbs). The MPG is based on the formation of a mixed precipitate composed of an antigen, the corresponding MAb and precipitating polyclonal antiserum. MAb incorporated into the precipitate is revealed by Fab'-peroxidase conjugate added to polyclonal antiserum. The MPG technique was applied to hybridoma screening as well as for the antigen and epitope specificity analysis of different MAbs. The MPG is a one-step, simple, inexpensive technique and valuable for the study of any antigen which could be revealed by immunodiffusion.

Download full-text PDF

Source
http://dx.doi.org/10.1016/0161-5890(87)90157-xDOI Listing

Publication Analysis

Top Keywords

mixed precipitation
8
precipitation gel
8
characterization monoclonal
8
monoclonal antibodies
8
mpg technique
8
mabs mpg
8
polyclonal antiserum
8
gel method
4
method identification
4
identification characterization
4

Similar Publications

Here, we report the design, synthesis, and comprehensive characterization of the bis-cholesterol supramolecular gelator, which contains photochromic stiff-stilbene as a bridging unit. The -isomer of stiff-stilbene bridged bis-cholesterol (-) was first synthesized with a systematic design, which can be further converted into its -isomer (-) with a high degree of efficiency (ca. 100%) upon exposure to 385 nm UV light.

View Article and Find Full Text PDF

The Liaohe River Estuary (LRE) wetland is a critical stopover on the East Asian-Australasian Flyway (EAAF), vital to coastal ecological balance and biodiversity. However, the drivers of changes in waterbird diversity remain unclear. This study utilised random forests to produce reliable time-series wetland mapping from 2010 to 2023.

View Article and Find Full Text PDF

Three different methods for ZnO-RGO nanocomposite synthesis and its adsorption capacity for methylene blue dye removal in a comparative study.

BMC Chem

January 2025

Environmental Applications of Nanomaterial's Lab., Department of Chemistry, Faculty of Science, Aswan University, Aswan, 81528, Egypt.

Water is one of the vital needs of life. However, due to rapid industrialization, urbanization and lack of awareness, the world population now facing the threat of water shortage. To ensure that future living conditions are preserved, it is crucial to reduce water pollution and protect the ecosystem.

View Article and Find Full Text PDF

The incorporation of ZIF-67 into hydrogels for wastewater pollutant remediation has been widely studied, but the synthesis often requires organic solvents such as methanol or ethanol, which can result in the generation of toxic liquid waste. In this study, a novel hydrogel (ZIF-67@SL) was synthesized by integrating ZIF-67 into a dual-network system of sodium lignosulfonate (SL) and acrylamide (AM) using an in situ precipitation method in water. The material was characterized by XRD, FTIR, XPS, SEM, TEM, BET, and TGA analyses.

View Article and Find Full Text PDF

The transition to net zero emissions requires the capture of carbon dioxide from industrial point sources, and direct air capture (DAC) from the atmosphere for geological storage. Dissolved CO has reactivity to rock core, and while the majority of previous studies have concentrated on reservoir rock or cap-rock reactivity, the underlying seal formation may also react with CO. Drill core from the underlying seal of a target CO storage site was reacted at in situ conditions with pure CO, and compared with an impure CO stream with SO, NO and O that could be expected from hard to abate industries.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!