AI Article Synopsis
- The hormonal action of jasmonate in plants is regulated by its production and breakdown, with cytochrome P450s CYP94B3 and CYP94C1 playing key roles in its inactivation.
- CYP94B3 not only helps in the inactivation of jasmonoyl-L-isoleucine but also catalyzes the hydroxylation of other jasmonoyl amino acids, leading to less active metabolites that accumulate after plant wounding.
- The study also identifies 12-O-β-glucopyranosyl-jasmonoyl-L-isoleucine as a new metabolite related to jasmonoyl-L-isoleucine, which rises in concentration following plant damage.
Article Abstract
The hormonal action of jasmonate in plants is controlled by the precise balance between its biosynthesis and inactivation. Oxidation of jasmonoyl-L-isoleucine at the C-12 position, which is catalyzed by cytochrome P450s CYP94B3 and CYP94C1, is thought to be one of the main inactivation pathways. In this study, an additional function of CYP94B3 was elucidated, as well additional jasmonoyl-L-isoleucine metabolites being investigated. It was found that CYP94B3 also catalyzes the hydroxylation of jasmonoyl-L-valine and jasmonoyl-L-phenylalanine, and that these hydroxyl compounds accumulated after wounding and possessed lower activity than non-hydroxylated compounds. Additionally, 12-O-β-glucopyranosyl-jasmonoyl-L-isoleucine accumulated after wounding, suggesting that it is a metabolite of jasmonoyl-L-isoleucine.
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| http://dx.doi.org/10.1016/j.phytochem.2013.12.019 | DOI Listing |
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