We performed a comparative study on the enzymological features of purified recombinant α-amylase of three species belonging to the Drosophila melanogaster species subgroup: D. melanogaster, D. erecta and D. sechellia. D. erecta and D. sechellia are specialist species, with host plant Pandanus candelabrum (Pandanaceae) and Morinda citrifolia (Rubiaceae), respectively. The temperature optima were around 57-60℃ for the three species. The pH optima were 7.2 for D. melanogaster, 8.2 for D. erecta and 8.5 for D. sechellia. The kcat and Km were also estimated for each species with different substrates. The specialist species D. erecta and D. sechellia display a higher affinity for starch than D. melanogaster. α-Amylase activity is higher on starch than on glycogen in all species. α-Amylases of D. erecta and D. sechellia have a higher activity on maltooligosaccharides (G6 and G7) than on starch, contrary to D. melanogaster. Such differences in the enzymological features between the species might reflect adaptation to different ecological niches and feeding habits.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1266/ggs.88.251 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Interrogating the 5'UTR tandem repeats of retrotransposon roo of Drosophila about horizontal transfer.
Genetica
June 2021
Departamento de Biología Funcional, Área de Genética, Universidad de Oviedo, 33071, Oviedo, Spain.
Horizontal transfer in Drosophila has been inferred for several families of transposable elements. Specifically, the retroelement roo has been suggested to have been horizontally transferred between the species D. melanogaster, D.
View Article and Find Full Text PDFDivergence of Drosophila species: Longevity and reproduction under different nutrient balances.
Genes Cells
September 2020
Graduate School of Biostudies, Kyoto University, Kyoto, Japan.
How nutrition impacts growth, reproduction and longevity is complex because relationships between these life events are difficult to disentangle. As a first step in sorting out these processes, we carried out a comparative analysis of related species of Drosophila with distinct feeding habits. In particular, we examined life spans and egg laying of two generalists and three specialists on diets with distinct protein-to-carbohydrate ratios.
View Article and Find Full Text PDFDomesticated Gene of LTR Retrotransposons Is Involved in Response to Oxidative Stress.
Genes (Basel)
April 2020
Department of Genetics, Faculty of Biology, Lomonosov Moscow State University, Moscow 119234, Russia.
is one of the most extensively used genetic model organisms for studying LTR retrotransposons that are represented by various groups in its genome. However, the phenomenon of molecular domestication of LTR retrotransposons has been insufficiently studied in , as well as in other invertebrates. The present work is devoted to studying the role of the domesticated gene, , in the genome.
View Article and Find Full Text PDFMolecular Logic and Evolution of Bitter Taste in Drosophila.
Curr Biol
January 2020
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA. Electronic address:
Taste systems detect a vast diversity of toxins, which are perceived as bitter. When a species adapts to a new environment, its taste system must adapt to detect new death threats. We deleted each of six commonly expressed bitter gustatory receptors (Grs) from Drosophila melanogaster.
View Article and Find Full Text PDFHighly Contiguous Genome Assemblies of 15 Species Generated Using Nanopore Sequencing.
G3 (Bethesda)
October 2018
Stowers Institute for Medical Research, Kansas City, MO.
The genus is a unique group containing a wide range of species that occupy diverse ecosystems. In addition to the most widely studied species, , many other members in this genus also possess a well-developed set of genetic tools. Indeed, high-quality genomes exist for several species within the genus, facilitating studies of the function and evolution of -regulatory regions and proteins by allowing comparisons across at least 50 million years of evolution.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!