Psoriatic and control human hair follicle keratinocytes were cultured on bovine eye lens capsules in Epicult dishes for a period of 5-6 weeks and examined using light microscopy. The following morphological differences between cultures were observed: 1. The lower cell layers contained predominantly flattened cells in psoriatic cultures instead of roundish in control cultures. 2. The differentiation pattern was irregular in psoriatic cultures instead of regular in control cultures. 3. The differentiated zone of psoriatic cultures was more compact and thicker in comparison to normal cultures. These differences might allow discrimination between normal and psoriatic cultures.
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- The study aimed to explore how inhibiting VEGF-A affects gene expression and pathways in both healthy and psoriatic skin samples.
- Results showed that VEGF-A inhibition led to changes in lipid metabolism and cell stress responses, indicating potential new avenues for psoriasis treatment, but further research is necessary to confirm these findings.
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