Inheritance of Trans Chromosomal Methylation patterns from Arabidopsis F1 hybrids.

Proc Natl Acad Sci U S A

Plant Industry Commonwealth Scientific and Industrial Research Organization, Canberra, ACT 2600, Australia.

Published: February 2014

AI Article Synopsis

  • Hybridization in plants can lead to interactions between parental genomes that change 24 nt siRNA and cytosine methylation (mC) levels in the resulting hybrids.
  • In Arabidopsis, two key processes—Trans Chromosomal Methylation (TCM) and Trans Chromosomal deMethylation (TCdM)—alter the methylation patterns inherited from parent plants, affecting gene expression in the hybrids.
  • The study found that specific genomic segments exhibit unique inheritance patterns, with TCM leading to reduced expression of certain genes, which may explain the decreased hybrid vigor seen in later generations.

Article Abstract

Hybridization in plants leads to transinteractions between the parental genomes and epigenomes that can result in changes to both 24 nt siRNA and cytosine methylation ((m)C) levels in the hybrid. In Arabidopsis the principle processes altering the hybrid methylome are Trans Chromosomal Methylation (TCM) and Trans Chromosomal deMethylation (TCdM) in which the (m)C pattern of a genomic segment attains the same (m)C pattern of the corresponding segment on the other parental chromosome. We examined two loci that undergo TCM/TCdM in the Arabidopsis C24/Landsberg erecta (Ler) F1 hybrids, which show patterns of inheritance dependent on the properties of the particular donor and recipient chromosomal segments. At At1g64790 the TCM- and TCdM-derived (m)C patterns are maintained in the F2 generation but are transmitted in outcrosses or backcrosses only by the C24 genomic segment. At a region between and adjacent to At3g43340 and At3g43350, the originally unmethylated Ler genomic segment receives the C24 (m)C pattern in the F1, which is then maintained in backcross plants independent of the presence of the parental C24 segment. In backcrosses to an unmethylated Ler allele, the newly methylated F1 Ler segment may act as a TCM source in a process comparable to paramutation in maize. TCM-derived (m)C patterns are associated with reduced expression of both At3g43340 and At3g43350 in F1 and F2 plants, providing support for such events influencing the transcriptome. The inheritance of the F1 (m)C patterns and the segregation of other genetic and epigenetic determinants may contribute to the reduced hybrid vigor in the F2 and subsequent generations.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3918825PMC
http://dx.doi.org/10.1073/pnas.1323656111DOI Listing

Publication Analysis

Top Keywords

trans chromosomal
12
genomic segment
12
chromosomal methylation
8
at3g43340 at3g43350
8
unmethylated ler
8
segment
6
patterns
5
inheritance trans
4
chromosomal
4
methylation patterns
4

Similar Publications

Autism spectrum disorder (ASD) is linked to ion channel dysfunction, including chloride voltage-gated channel-4 (CLCN4). We generated Clcn4 knockout (KO) mice by deleting exon 5 of chromosome 7 in the C57BL/6 mice. Clcn4 KO exhibited reduced social interaction and increased repetitive behaviors assessed using three-chamber and marble burying tests.

View Article and Find Full Text PDF

Background: Acute promyelocytic leukemia (APL) is characterized by abnormal promyelocytes and t(15;17)(q24;q21) . Rarely, patients may have cryptic or variant rearrangements. All-trans retinoic acid (ATRA)/arsenic trioxide (ATO) is largely curative provided that the diagnosis is established early.

View Article and Find Full Text PDF

Chimerism: A whole new perspective in gene regulation.

Biochim Biophys Acta Gen Subj

January 2025

National Centre for Cell Science, Savitribai Phule Pune University, Ganeshkhind, Pune 411007, India. Electronic address:

The diversity of molecular entities emerging from a single gene are recognized. Several studies have thus established the cellular role(s) of transcript variants and protein isoforms. A step ahead in challenging the central dogma towards expanding molecular diversity is the identification of fusion genes, chimeric transcripts and chimeric proteins that harbor sequences from more than one gene.

View Article and Find Full Text PDF

Background: There is increasing need for effective incorporation of high-dimensional genetics data from individuals with varied ancestry in genome-wide association (GWAS) analyses. Classically, multi-ancestry GWAS analyses are performed using statistical meta-analysis to combine results conducted within homogeneous ancestry groups. The emergence of cosmopolitan reference panels makes collective preprocessing of GWAS data possible, but impact on downstream GWAS results in a mega-analysis framework merits investigation.

View Article and Find Full Text PDF

Comprehensive genome annotation of Trilocha varians, a new model species of Lepidopteran insects.

Sci Data

January 2025

Gakushuin University, Faculty of Science, Department of Life Science, Mejiro 1-5-1, Toshima-ku, Tokyo, 171-8588, Japan.

Trilocha varians is a member of the bombycid moths. Since T. varians has a considerably shorter generation period than the prevailing model species, Bombyx mori, this species would be a novel model insect in Lepidoptera.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!