AI Article Synopsis
- The study investigates the role of the hypothalamic paraventricular nucleus (PVN) as a key autonomic control center in the brain, particularly focusing on how certain prostanoids like PGE2 influence its function.
- Researchers employed liquid chromatography ion trap tandem mass spectrometry (LC-ITMS(n)) to measure levels of four specific prostanoids in microdialysates from urethane-anesthetized rats, establishing precise quantification limits and high reproducibility.
- The findings suggest that thromboxane (Tx) A2 may significantly influence the adrenomedullary response triggered by neurotransmitters, marking the first use of LC-ITMS(n) for this type of analysis in PVN studies
Article Abstract
The hypothalamic paraventricular nucleus (PVN) is one of the most important autonomic control centers in the brain. Several kinds of prostanoids, such as prostaglandin (PG) E2, are considered to act in the PVN as mediators of autonomic responses. In the present study, we used liquid chromatography ion trap tandem mass spectrometry (LC-ITMS(n)) to simultaneously quantify four prostanoids, thromboxane (Tx) B2, PGE2, PGD2 and 15-deoxy-∆(12,14) (15d)-PGJ2 in PVN microdialysates from urethane-anesthetized rats. The quantification limits were estimated to be 0.05ng/mL for TxB2, 0.025ng/mL for PGE2, 0.1ng/mL for PGD2, and 0.5ng/mL for 15d-PGJ2. The RSD% obtained from all prostanoids was <15%, indicating an acceptable level of reproducibility. LC-ITMS(n) analysis of rat PVN microdialysates revealed that TxA2 may play an important role in adrenomedullary outflow evoked by centrally administered N-methyl-d-aspartate, corticotrophin-releasing factor and glucagon-like peptide-1. This is the first study to use LC-ITMS(n) to analyze prostanoid levels in rat PVN microdialysates. This LC-ITMS(n) method will be useful for investigating the potential involvement of prostanoids in brain function.
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| http://dx.doi.org/10.1016/j.autneu.2013.12.013 | DOI Listing |
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