Inhibition of KRAS-driven tumorigenicity by interruption of an autocrine cytokine circuit.

Cancer Discov

Departments of 1Medical Oncology and 2Cancer Biology, Dana-Farber Cancer Institute; 3Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston; 4Broad Institute of Harvard and MIT, Cambridge; 5MGH Cancer Center, 6Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Charlestown, Massachusetts; and 7Department of Surgery, Division of Biology and Biomedical Sciences, Washington University, St. Louis, Missouri.

Published: April 2014

AI Article Synopsis

  • KRAS, a key player in tumor development, activates additional pathways beyond MAPK and PI3K, which are crucial for tumor maintenance.
  • Researchers found that the kinases TBK1 and IKKε support KRAS-driven tumor growth by regulating the release of cytokines like CCL5 and IL-6.
  • The drug CYT387 effectively inhibits these signaling pathways, leading to reduced tumor growth and enhanced treatment effects when combined with MAPK inhibition in mouse models of lung cancer.

Article Abstract

Although the roles of mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) signaling in KRAS-driven tumorigenesis are well established, KRAS activates additional pathways required for tumor maintenance, the inhibition of which are likely to be necessary for effective KRAS-directed therapy. Here, we show that the IκB kinase (IKK)-related kinases Tank-binding kinase-1 (TBK1) and IKKε promote KRAS-driven tumorigenesis by regulating autocrine CCL5 and interleukin (IL)-6 and identify CYT387 as a potent JAK/TBK1/IKKε inhibitor. CYT387 treatment ablates RAS-associated cytokine signaling and impairs Kras-driven murine lung cancer growth. Combined CYT387 treatment and MAPK pathway inhibition induces regression of aggressive murine lung adenocarcinomas driven by Kras mutation and p53 loss. These observations reveal that TBK1/IKKε promote tumor survival by activating CCL5 and IL-6 and identify concurrent inhibition of TBK1/IKKε, Janus-activated kinase (JAK), and MEK signaling as an effective approach to inhibit the actions of oncogenic KRAS.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3980023PMC
http://dx.doi.org/10.1158/2159-8290.CD-13-0646DOI Listing

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