AI Article Synopsis
- The study introduces a protocol for the scalable automated growth of human induced pluripotent stem cells in a defined medium, crucial for large-scale production of cell therapies.
- The research successfully demonstrates the transfer of stem cells between lab and manufacturing settings, utilizing both manual and automated methods.
- Key findings highlight that managing cell aggregates is vital, with comparisons made on cellular characteristics and gene expression between the two processes.
Article Abstract
The transfer of a laboratory process into a manufacturing facility is one of the most critical steps required for the large scale production of cell-based therapy products. This study describes the first published protocol for scalable automated expansion of human induced pluripotent stem cell lines growing in aggregates in feeder-free and chemically defined medium. Cells were successfully transferred between different sites representative of research and manufacturing settings; and passaged manually and using the CompacT SelecT automation platform. Modified protocols were developed for the automated system and the management of cells aggregates (clumps) was identified as the critical step. Cellular morphology, pluripotency gene expression and differentiation into the three germ layers have been used compare the outcomes of manual and automated processes.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3969287 | PMC |
| http://dx.doi.org/10.1016/j.jbiotec.2013.12.009 | DOI Listing |
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