AI Article Synopsis
- The study recorded electrical responses from nerve endings in frog muscles, focusing on the effects of different solutions on these responses.
- When a Ca2+-free solution and tubocurarine were applied, a late inward current was observed that transformed into an outward current when 4-aminopyridine was introduced.
- The results suggest that the late inward current is through calcium channels, while the late outward current is through calcium-activated potassium channels, influencing the release of neurotransmitters.
Article Abstract
Evoked electrical responses of nerve endings were recorded in experiments on frog cutaneous-pectoris muscle under visual control. During superfusion by Ca2+-free solution with tubocurarine a late inward current was found in the evoked response of nerve endings recorded by CaCl2 filled electrode. With addition of the external solution of 4-aminopyridine the late current was changed into an outward one. This outward current depended on Ca2+ concentration in the electrode, decreased after local increase of K+ concentration and was abolished by Co2+ ions. Local ionophoretic application of tetraethyl-ammonium eliminated the outward current and revealed a strong and prolonged inward current. Similar currents were recorded by microelectrodes filled with SrCl2, BaCl2 and MgCl2. It is concluded that the late inward current is carried through potential-dependent calcium channels, while the late outward one--through Ca2+-activated K+ channels. The ratio of calcium and Ca2+-activated potassium currents in motor nerve endings and their role in the transmitter release are discussed.
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