To know the genetic structure and phylogeny status, the complete mitochondrial genome of Thitarodes pui was the first time to be sequenced. The genome was 15,064 bp in length and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, as well as an AT rich region. The base composition of the genome was A (40.93%), T (39.80%), C (11.72%) and G (7.55%), with an AT content of 80.73%. It is interesting to that the COI would start with CGA. The tRNA order between ND2 and AT rich region was tRNA(Ile)-tRNA(Gln)-tRNA(Met), which confirms the thesis that Thitarodes has the ancestral gene arrangement. It is surprised that AT-rich region was 287 bp in length and became the shortest in contrast to other insects of Hepialidae.
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