In this study, we determined the complete nucleotide sequence of the mitochondrial genome for a cold water fish, Leuciscus idus. The complete mitochondrial genome was sequenced and analyzed using the primer walking method and 30 pairs of newly designed primers were used. Direct sequencing of the PCR products demonstrated that the genome contained the same 37 mitochondrial genes (two ribosomal RNA, 22 transfer RNA and 13 protein-coding genes) and two main non-coding regions (the control region and the origin of the light strand replication) as those found in other vertebrates. Nearly all the protein-coding genes' start codons were ATG except COX 1, meanwhile, the termination codons of which varied with TAA, TA, T, or TAG.
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| http://dx.doi.org/10.3109/19401736.2013.873924 | DOI Listing |
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